製品の概要

  • 製品名
    Anti-Fluorescein antibody [6A4]
    Fluorescein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [6A4] to Fluorescein
  • アプリケーション
    適用あり: WB, ELISA, IP, ICCmore details
  • 免疫原

    Synthetic peptide corresponding to Fluorescein.

  • 特記事項
    Human synaptosome associated protein 23 (SNAP23) MW is 27 kDa

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Constituents: 0.75% Glycine, 1.21% Tris, 2% Sucrose
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    6A4
  • ミエローマ
    unknown
  • アイソタイプ
    IgM
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/5000.
ELISA 1/2000 - 1/20000.
IP Use at an assay dependent concentration. PubMed: 21857021
ICC 1/200 - 1/2000.

ターゲット情報

  • 関連性
    Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
  • 別名
    • FI antibody
    • FITC antibody
    • fluorescein isothyocianate antibody
    • fluorescyl antibody

参考文献

This product has been referenced in:
  • Maheswaranathan M  et al. Platelet-derived Growth Factor (PDGF) Regulates Slingshot Phosphatase Activity via Nox1-dependent Auto-dephosphorylation of Serine 834 in Vascular Smooth Muscle Cells. J Biol Chem 286:35430-7 (2011). IP . Read more (PubMed: 21857021) »
  • Odagi I  et al. Examination of normal intestine using confocal endomicroscopy. J Gastroenterol Hepatol 22:658-62 (2007). Read more (PubMed: 17444852) »

See all 2 Publications for this product

レビューと Q&A

Thank you for your enquiry.

I am sorry to confirm that regrettablythese antibodies have not yet been tested and guaranteed in this type of ELISAcoating the antibody on theplate using protein A. They have been testedas detection antibodies in...

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Thank you for your enquiry. I am sorry that the answer has taken so long. The answer from the source of the antiserum has not been forthcoming. Unfortunately I have been informed that ab6213's cross reactivity with carboxyfluorescein has never been...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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