製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Flt3 ligand
  • アプリケーション
    適用あり: WB, Neutralising, Flow Cyt, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hflt3-Ligand (human flt3-Ligand)

  • 特記事項


    hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 一次抗体 備考
    hflt3-Ligand (flk2-ligand) stimulates the proliferation and colony formation of certain bone marrow precursor cells including CD34+ cells
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    unknown
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.2 µg/ml. The detection limit for recombinant Flt3 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Neutralising Use a concentration of 0.004 - 0.006 µg/ml. Yields one-half maximal inhibition [ND50] of the biological activity of hFlt-3 Ligand (1.0 ng/ml)
Flow Cyt Use at an assay dependent concentration. PubMed: 22897934
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. In conjunction with ab84261 rabbit polyclonal to Flt3 ligand (Biotin) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFlt3 Ligand.

ターゲット情報

  • 機能
    Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FL antibody
    • Flt 3 ligand antibody
    • Flt 3L antibody
    • Flt3 L antibody
    • FLT3 LG antibody
    • Flt3 ligand antibody
    • Flt3L antibody
    • FLT3L_HUMAN antibody
    • Flt3lg antibody
    • Fms related tyrosine kinase 3 ligand antibody
    • Fms-related tyrosine kinase 3 ligand antibody
    • SL cytokine antibody
    see all

画像

  • Flow cytometry analysis of Human Flt3 ligand-transfected mouse OP9 cell line, staining Flt3 ligand with ab9688. The primary antibody was conjugated to AlexaFluor®488 before incubation with paraformaldehyde-fixed, serum-blocked cells.

参考文献

This product has been referenced in:
  • Patel ES  et al. Regulation of in vitro human T cell development through interleukin-7 deprivation and anti-CD3 stimulation. BMC Immunol 13:46 (2012). Flow Cyt ; Human . Read more (PubMed: 22897934) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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