Anti-Flt3 / CD135 抗体 - Aminoterminal end (ab37847)

製品の概要

  • 製品名
    Anti-Flt3 / CD135 antibody - Aminoterminal end
    Flt3 / CD135 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Flt3 / CD135 - Aminoterminal end
  • アプリケーション
    適用あり: ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from aa 48-65 (

    GKSSSYPMVSESPEDLGC

    ) of human Flt3/ CD135.

  • ポジティブ・コントロール
    • HL-60 cell lysate. breast carcinoma This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab37847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt 1/1 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.
  • 組織特異性
    Bone marrow cells.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD 135 antibody
    • CD135 antibody
    • CD135 antigen antibody
    • Fetal liver kinase 2 antibody
    • FL cytokine receptor antibody
    • Flk 2 antibody
    • Flk2 antibody
    • Flt 3 antibody
    • FLT-3 antibody
    • Flt3 antibody
    • FLT3_HUMAN antibody
    • FMS like tyrosine kinase 3 antibody
    • Fms related tyrosine kinase 3 antibody
    • Fms-like tyrosine kinase 3 antibody
    • Growth factor receptor tyrosine kinase type III antibody
    • Ly-72 antibody
    • OTTHUMP0000004234 antibody
    • Receptor type tyrosine protein kinase FLT3 antibody
    • Stem cell tyrosine kinase 1 antibody
    • Stk 1 antibody
    • STK-1 antibody
    • Stk1 antibody
    • Tyrosine protein kinase receptor FLT3 antibody
    • Tyrosine-protein kinase receptor FLT3 antibody
    see all

Anti-Flt3 / CD135 antibody - Aminoterminal end 画像

  • Flow cytometric analysis of 293 cells, staining Flt3 / CD135 (green) with ab37847 at 1/10 dilution. A FITC-conjugated goat-anti-rabbit secondary was used.

  • Flow Cytometry analysis of Flt3 / CD135 in human endothelial precursor cells using ab37847 undiluted. The secondary used was an undiluted Allophycocyanin conjugated goat anti-rabbit polyclonal.
    Red line= ab37847
    Black line= isotype control

    See Abreview

  • ab37847 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37847 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Flt3 / CD135 antibody - Aminoterminal end (ab37847) 使用論文

This product has been referenced in:
  • Jackson AM  et al. Endothelial cell antibodies associated with novel targets and increased rejection. J Am Soc Nephrol 26:1161-71 (2015). Read more (PubMed: 25381426) »

See 1 Publication for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (endothelial precursor cell)
Specification
endothelial precursor cell
Fixation
none
Permeabilization
No
Gating Strategy
CD133 and CD202B
Username

Dr. A Jackson

Verified customer

投稿 Dec 05 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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