製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Flotillin 2
  • アプリケーション
    適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to a region within the C terminal amino acids 365-428 of Human Flotillin 2.

  • ポジティブ・コントロール
    • HeLa S3 cell lysate, Lung CA (IHC)

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is not essential but may optimise staining.
ICC/IF 1/100 - 1/200.
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
  • 組織特異性
    In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
  • 配列類似性
    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • 細胞内局在
    Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ECS-1 antibody
    • ECS1 antibody
    • Epidermal surface antigen 1 antibody
    • Epidermal surface antigen antibody
    • ESA antibody
    • ESA1 antibody
    • Flot2 antibody
    • FLOT2_HUMAN antibody
    • Flotillin 2 (epidermal surface antigen 1) antibody
    • Flotillin-2 antibody
    • M17S1 antibody
    • Membrane component chromosome 17 surface marker 1 antibody
    • Membrane component chromosome 17 surface marker 1 homolog antibody
    • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1) antibody
    • Membrane component, chromosome 17, surface marker 1 antibody
    • REG-1 antibody
    • Reggie-1 antibody
    • Reggie-2 antibody
    see all

画像

  • Anti-Flotillin 2 antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg

    Predicted band size : 47 kDa
  • Immunohistochemical analysis of paraffin-embedded Lung CA, using ab96507 at 1:100 dilution.
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
  • Flotillin 2 immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.

    Lane 1 : anti-Flotillin 2 IP (using non-Abcam antibody)
    Lane 2 : anti-Flotillin 2 IP (using ab96507)
    Lane 3 : Input (HeLa cell lysate)
    Lane 4 : Supernatant IP (using non-Abcam antibody)
    Lane 5 : Supernatant IP (using ab96507)

    Western blot detection : mouse anti-Flotillin 2 antibody + HRP donkey anti-mouse polyclonal secondary antibody

    See Abreview

参考文献

This product has been referenced in:
  • Gong J  et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). Read more (PubMed: 27354374) »
  • Zhu Q  et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . Read more (PubMed: 26443540) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (HeLa)
Total protein in input
750 µg
Specification
HeLa
Immuno-precipitation step
Protein A
Username

Prof. Ritva Tikkanen

Verified customer

投稿 Jan 11 2013

I am very pleased to hear you would like to test both ab41927 and ab96507 in immunoprecipitation. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for immunoprecipitation and in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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