Anti-Flotillin 2/ESA 抗体 (ab96507)
Key features and details
- Rabbit polyclonal to Flotillin 2/ESA
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Flotillin 2/ESA antibody
Flotillin 2/ESA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Flotillin 2/ESA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Flotillin 2/ESA aa 365-428 (C terminal).
Database link: Q14254 -
ポジティブ・コントロール
- WB: HeLa. Mouse brain lysate. Rat brain lysate. IP: Human HeLa whole cell lysate. IHC-P: human lung cancer. ICC/IF: HeLa S3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96507の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
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IHC-P |
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
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ICC/IF |
1/100 - 1/1000.
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IP | (1) |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 47 kDa. |
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. |
ICC/IF
1/100 - 1/1000. |
IP
1/100 - 1/500. |
ターゲット情報
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機能
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function. -
組織特異性
In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes. -
配列類似性
Belongs to the band 7/mec-2 family. Flotillin subfamily. -
細胞内局在
Cell membrane. Membrane > caveola. Endosome. Membrane-associated protein of caveolae. - Information by UniProt
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参照データベース
- Entrez Gene: 2319 Human
- Entrez Gene: 14252 Mouse
- Entrez Gene: 83764 Rat
- Omim: 131560 Human
- SwissProt: Q14254 Human
- SwissProt: Q60634 Mouse
- SwissProt: Q9Z2S9 Rat
- Unigene: 514038 Human
see all -
別名
- ECS-1 antibody
- ECS1 antibody
- Epidermal surface antigen 1 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human lung cancer using ab96507 at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 47 kDa -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa S3 cells, using ab96507 at 1:100 dilution. Image 2: Merged with DNA probe.
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Flotillin 2 was immunoprecipitated from 5µ of HeLa whole cell lysate with ab96507. Western blot was performed from the immunoprecipitate using ab96507. EasyBlot anti-Rabbit IgG, was used as secondary antibody.
Lane 1: HeLa lysate 5µg (Input).
Lane 2: ab96507 IP in HeLa lysate. -
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + Rat brain lysate at 50 µg
Predicted band size: 47 kDa10% SDS-PAGE
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Flotillin 2/ESA immunoprecipitation from Human HeLa whole cell lysate using ab96507. 750 µg of cell lysate was incubated with primary antibody (1/500) and matrix (Protein A) and incubated for 16 hours at 4°C.
Lane 1 : anti-Flotillin 2/ESA IP (using non-Abcam antibody)
Lane 2 : anti-Flotillin 2/ESA IP (using ab96507)
Lane 3 : Input (HeLa cell lysate)
Lane 4 : Supernatant IP (using non-Abcam antibody)
Lane 5 : Supernatant IP (using ab96507)
Western blot detection : mouse anti-Flotillin 2/ESA antibody + HRP donkey anti-mouse polyclonal secondary antibody -
Anti-Flotillin 2/ESA antibody (ab96507) at 1/1000 dilution + HeLa S3 whole cell lysate at 30 µg
Predicted band size: 47 kDa
10% SDS PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab96507 は 6 報の論文で使用されています。
- Biernatowska A et al. MPP1 Determines the Mobility of Flotillins and Controls the Confinement of Raft-Associated Molecules. Cells 11:N/A (2022). PubMed: 35159121
- Bai C et al. Cryopreservation disrupts lipid rafts and heat shock proteins in yellow catfish sperm. Cryobiology 87:32-39 (2019). PubMed: 30876909
- Li T et al. FLOT2 overexpression is associated with the progression and prognosis of human colorectal cancer. Oncol Lett 17:2802-2808 (2019). PubMed: 30854055
- Gong J et al. Exosomes mediate cell contact-independent ephrin-Eph signaling during axon guidance. J Cell Biol 214:35-44 (2016). PubMed: 27354374
- Zhu Q et al. Overexpression of acylglycerol kinase is associated with poorer prognosis and lymph node metastasis in nasopharyngeal carcinoma. Tumour Biol 37:3349-57 (2016). WB, IHC . PubMed: 26443540
- Wang X et al. Flotillin-2 is associated with breast cancer progression and poor survival outcomes. J Transl Med 11:190 (2013). WB . PubMed: 23945257