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RabMAb

Anti-Flotillin 1 抗体 [EP446Y] (ab78178)

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製品の概要

  • 製品名
    Anti-Flotillin 1 antibody [EP446Y]
    Flotillin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP446Y] to Flotillin 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Flotillin 1 aa 50-150.
    Database link: O75955

  • ポジティブ･コントロール
    • K562 cell lysate. C2C12 cell lysate.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 48 kDa.

For unpurified, use 1/500 - 1/1000.

ターゲット情報

  • 機能
    May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • 配列類似性
    Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • 細胞内局在
    Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
    • 別名
      • FLOT 1 antibody
      • FLOT1 antibody
      • FLOT1_HUMAN antibody
      • Flotillin-1 antibody
      • Flotillin1 antibody
      • Integral membrane component of caveolae antibody
      • Reggie 2 antibody
      see all

    画像

    • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Flotillin 1 knockout HAP1 whole cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab78178 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab78178 was shown to specifically react with Flotillin 1 in wild-type HAP1 cells as signal was lost in Flotillin 1 knockout cells. Wild-type and Flotillin 1 knockout samples were subjected to SDS-PAGE. ab78178 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

      Lane 1 : mouse brain lysate at 10 µg
      Lane 2 : rat brain lysate at 20 µg
      Lane 3 : C6 cell lysate at 20 µg

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 48 kDa
      Observed band size: 49 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

      Lane 1 : K562 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

      Predicted band size: 48 kDa
      Observed band size: 49 kDa (why is the actual band size different from the predicted?)



      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      Western blot - Anti-Flotillin 1 antibody [EP446Y] (ab78178)
      Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution (unpurified) + K562 cell lysate

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    プロトコール

    参考文献

    ab78178 has not yet been referenced specifically in any publications.

    ab78178 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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