製品の概要

  • 製品名Anti-Flotillin 1 antibody [EP446Y]
    Flotillin 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP446Y] to Flotillin 1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Flotillin 1 aa 50-150.
    Database link: O75955

  • ポジティブ・コントロール
    • K562 cell lysate. C2C12 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 48 kDa.

For unpurified, use 1/500 - 1/1000.

ターゲット情報

  • 機能May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
  • 配列類似性Belongs to the band 7/mec-2 family. Flotillin subfamily.
  • 細胞内局在Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLOT 1 antibody
    • FLOT1 antibody
    • FLOT1_HUMAN antibody
    • Flotillin-1 antibody
    • Flotillin1 antibody
    • Integral membrane component of caveolae antibody
    • Reggie 2 antibody
    see all

Anti-Flotillin 1 antibody [EP446Y] 画像

  • All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

    Lane 1 : mouse brain lysate at 10 µg
    Lane 2 : rat brain lysate at 20 µg
    Lane 3 : C6 cell lysate at 20 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/2000 dilution (purified)

    Lane 1 : K562 cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 48 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Flotillin 1 antibody [EP446Y] (ab78178) at 1/500 dilution (unpurified) + K562 cell lysate

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

Anti-Flotillin 1 antibody [EP446Y] (ab78178) 使用論文

ab78178 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab78178.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"