保存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH: 7-8
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精製度 Immunogen affinity purified
特記事項（精製） ab123512 was affinity purified using an epitope specific to Flotillin 1 immobilized on a solid support.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
機能 May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
配列類似性 Belongs to the band 7/mec-2 family. Flotillin subfamily.
細胞内局在 Cell membrane. Membrane > caveola. Melanosome. Endosome. Membrane-associated protein of caveolae. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Information by UniProt
FLOT 1 antibody
Anti-Flotillin 1 antibody 画像
Immunoprecipitation - Anti-Flotillin 1 antibody (ab123512)
Detection of Flotillin 1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab123512 at 6µg/mg lysate for IP (Lane 1). For WB detection an anti-Flotillin 1 antibody which recognizes an downstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
Anti-Flotillin 1 antibody (ab123512) 使用論文
has not yet been referenced specifically in any publications.
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