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Our Abpromise guarantee covers the use of ab8421 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 55 kDa.Can be blocked with Human FLIP peptide (ab8457).|
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemistry of FLIP in mouse liver tissue with FLIP antibody at 5 µg/ml.
ab8421 at 5µg/ml staining FLIP in Hela cells by ICC/IF