製品の概要

  • 製品名Anti-FLIP antibody
    FLIP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FLIP
  • 特異性This antibody recognises the 55kD FLIPL (FLIP alpha) protein.
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 447-464 of Human FLIP.

  • ポジティブ・コントロール
    • HeLa cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab6144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with FLIP peptide (ab8129).

ターゲット情報

  • 機能Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
  • 組織特異性Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
  • 配列類似性Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • ドメインThe caspase domain lacks the active sites residues involved in catalysis.
  • 翻訳後修飾Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c FLIP antibody
    • c FLIPL antibody
    • c FLIPR antibody
    • c FLIPS antibody
    • c-FLIP antibody
    • c-FLIPL antibody
    • c-FLIPR antibody
    • c-FLIPS antibody
    • CASH antibody
    • CASP8 and FADD like apoptosis regulator antibody
    • CASP8 and FADD-like apoptosis regulator subunit p12 antibody
    • CASP8AP1 antibody
    • Caspase homolog antibody
    • Caspase like apoptosis regulatory protein antibody
    • Caspase related inducer of apoptosis antibody
    • Caspase-eight-related protein antibody
    • Caspase-like apoptosis regulatory protein antibody
    • Casper antibody
    • Cellular FLICE-like inhibitory protein antibody
    • CFLA antibody
    • Cflar antibody
    • CFLAR_HUMAN antibody
    • CLARP antibody
    • FADD like anti apoptotic molecule antibody
    • FADD-like antiapoptotic molecule 1 antibody
    • FLAME antibody
    • FLAME-1 antibody
    • FLAME1 antibody
    • FLIP antibody
    • I FLICE antibody
    • I-FLICE antibody
    • Inhibitor of FLICE antibody
    • MACH-related inducer of toxicity antibody
    • MRIT antibody
    • OTTHUMP00000163715 antibody
    • OTTHUMP00000206475 antibody
    • OTTHUMP00000206476 antibody
    • OTTHUMP00000206478 antibody
    • OTTHUMP00000206479 antibody
    • OTTHUMP00000206480 antibody
    • OTTHUMP00000206482 antibody
    • OTTHUMP00000207360 antibody
    • Usurpin antibody
    • Usurpin beta antibody
    see all

Anti-FLIP antibody 画像

  • All lanes : Anti-FLIP antibody (ab6144) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : K562 whole cell lysate


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • ab6144 at 10µg/ml staining FLIP in Jurkat cells by ICC/IF

  • Immunofluorescence of FLIP in Jurkat cells using ab6144 at 10 ug/ml.

Anti-FLIP antibody (ab6144) 使用論文

This product has been referenced in:
  • Siu PM  et al. Response of XIAP, ARC, and FLIP apoptotic suppressors to 8 wk of treadmill running in rat heart and skeletal muscle. J Appl Physiol 99:204-9 (2005). WB ; Rat . Read more (PubMed: 15774698) »
  • Siu PM  et al. Aging sustains the hypertrophy-associated elevation of apoptotic suppressor X-linked inhibitor of apoptosis protein (XIAP) in skeletal muscle during unloading. J Gerontol A Biol Sci Med Sci 60:976-83 (2005). Read more (PubMed: 16127099) »

See all 2 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Rat Tissue lysate - whole (Eskeletal muscle)
Loading amount 30 µg
Specification Eskeletal muscle
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 May 27 2009

Yes, this antibody will recognise the human form. To our knowledge, ab6144 has not been tested in immunoblotting.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"