Anti-FLI1 抗体 [EPR4645] (ab124791)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4645] to FLI1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FLI1 antibody [EPR4645]
FLI1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4645] to FLI1 -
由来種
Rabbit -
特異性
This product does not cross-react with ERG. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human FLI1 aa 50-150. The exact sequence is proprietary.
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ポジティブ・コントロール
- Raw264.7, U937, Jurkat, K562 and C6 cell lysates. FLI1 recombinant protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR4645 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124791の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 51 kDa. |
ターゲット情報
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機能
Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'. -
関連疾患
Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1. -
配列類似性
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Contains 1 PNT (pointed) domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2313 Human
- Entrez Gene: 14247 Mouse
- Entrez Gene: 315532 Rat
- Omim: 193067 Human
- SwissProt: Q01543 Human
- SwissProt: P26323 Mouse
- Unigene: 504281 Human
- Unigene: 258908 Mouse
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別名
- ERGB transcription factor antibody
- Ewing Sarcoma breakpoint region 2 antibody
- EWSR 2 antibody
see all
画像
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All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution
Lane 1 : Raw264.7 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lane 5 : C6 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa -
All lanes : Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution
Lane 1 : ERG recombinant protein
Lane 2 : FLI1 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 51 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab124791 は 1 報の論文で使用されています。
- Ross NT et al. CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma. Nat Chem Biol 16:50-59 (2020). PubMed: 31819276