製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FLI1
  • 特異性
    FLI1 antibody detects endogenous levels of total FLI1 protein.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide derived from human FLI1

  • ポジティブ・コントロール
    • human breast carcinoma tissue; NIH/3T3 cell extract

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.

ターゲット情報

  • 機能
    Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 関連疾患
    Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • 配列類似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • Fli-1 proto-oncogene, ETS transcription factor antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 (FLI1) transcription factor antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • Sarcoma breakpoint region 2 (EWSR2) antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    • Viral integration region FLI1, mouse, homolog of antibody
    see all

画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53077 at 1/50 dilution, with and without immunizing peptide.
  • All lanes : Anti-FLI1 antibody (ab53077) at 1/50 dilution

    Lane 1 : NIH/3T3 cell extract with no immunizing peptide
    Lane 2 : NIH/3T3 cell extract with immunizing peptide


    Predicted band size : 51 kDa
    Observed band size : 51 kDa

参考文献

ab53077 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification
haematopoietic progenitor cell line HPC-7
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: both 0.4% and 1% formaldehyde
Detection step
Real-time PCR
Positive control
i) a competitors antibody known to work
ii) regulatory region known to bind this transcription factor
Negative control
a known region that this transcription factor does not bind
Username

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投稿 Oct 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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