製品の概要

  • 製品名Anti-FLI1 antibody
    FLI1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FLI1
  • 特異性This antibody recognizes FLI1
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog
  • 免疫原

    A region within synthetic peptide: SGSPDYGQPH KINPLPPQQE WINQPVRVNV KREYDHMNGS RESPVDCSVS, corresponding to N terminal amino acids 37-86 of Human FLI1

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab49045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/312500.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
  • 関連疾患Defects in FLI1 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving FLI1 is found in patients with Erwing sarcoma. Translocation t(11;22)(q24;q12) with EWSR1.
  • 配列類似性Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
    Contains 1 PNT (pointed) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ERGB transcription factor antibody
    • Ewing Sarcoma breakpoint region 2 antibody
    • EWSR 2 antibody
    • EWSR2 antibody
    • FLI 1 antibody
    • FLI 1 proto oncogene antibody
    • FLI1 antibody
    • FLI1 EWS fusion gene antibody
    • FLI1 proto oncogene antibody
    • FLI1_HUMAN antibody
    • Friend leukemia integration 1 transcription factor antibody
    • Friend leukemia virus integration 1 antibody
    • Proto-oncogene Fli-1 antibody
    • SIC 1 antibody
    • SIC1 antibody
    • Transcription factor ERGB antibody
    • Viral integration region FLI1 antibody
    see all

Anti-FLI1 antibody 画像

  • Lane 1 : MARKER
    Lane 2 : Anti-FLI1 antibody (ab49045) at 1.25 µg/ml

    Lane 1 : As above
    Lane 2 : Jurkat cell lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 51 kDa
    Observed band size : 51 kDa
  • All lanes : Anti-FLI1 antibody (ab49045) at 1/1000 dilution

    Lane 1 : Cytoplasmic lysate prepared from porcine endothelial cells
    Lane 2 : Nuclear lysate prepared from porcine endothelial cells

    Secondary
    Biotin conjugated anti-rabbit at 1/1000 dilution
    Developed using the ECL technique

    Predicted band size : 51 kDa


    Exposure time : 1 minute

Anti-FLI1 antibody (ab49045) 使用論文

ab49045 has not yet been referenced specifically in any publications.

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