製品の概要

  • 製品名
    Anti-FLG2 antibody
  • 製品の詳細
    Rabbit polyclonal to FLG2
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    amino acids 280-381 of Human FLG2 (Q5D862).

  • ポジティブ・コントロール
    • Human prostate tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 配列類似性
    Belongs to the S100-fused protein family.
    In the N-terminal section; belongs to the S-100 family.
    Contains 2 EF-hand domains.
    Contains 10 filaggrin repeats.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FILA2_HUMAN antibody
    • Filaggrin-2 antibody
    • FLG-2 antibody
    • FLG2 antibody
    • Ifapsoriasin antibody
    • Intermediate filament-associated and psoriasis-susceptibility protein antibody
    see all

Anti-FLG2 antibody 画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab122011 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122011, staining FLG2 in paraffin-embedded Human prostate tissue by Immunohistochemistry.

Anti-FLG2 antibody (ab122011) 使用論文

ab122011 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Loading amount
14 µg
Gel Running Conditions
Reduced Denaturing (gel 10%)
Sample
Human Serum (plasma)
Specification
plasma
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Miss. Yi-Tsen Chiang

Verified customer

投稿 Jan 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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