製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to FLAP
  • アプリケーション
    適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rabbit, Horse, Cynomolgus monkey, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-HKVEHESRTQNGR

    , corresponding to internal sequence amino acids 28-40 of Human FLAP

  • ポジティブ・コントロール
    • U937 or Peripheral Blood Mononucleocyte lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 6 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).

ターゲット情報

  • 機能
    Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.
  • 関連疾患
    Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
    Note=Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition.
  • 配列類似性
    Belongs to the MAPEG family.
  • ドメイン
    The C-terminal part after residue 140 is mostly unstructured.
  • 細胞内局在
    Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5-lipoxygenase activating protein antibody
    • AL5AP_HUMAN antibody
    • ALOX 5AP antibody
    • ALOX5 AP antibody
    • ALOX5AP antibody
    • Arachidonate 5 lipoxygenase activating protein antibody
    • Arachidonate 5-lipoxygenase-activating protein antibody
    • Five lipoxygenase activating protein antibody
    • FLAP antibody
    • MK 886 binding protein antibody
    • MK-886-binding protein antibody
    see all

画像

  • Anti-FLAP antibody (ab53536) at 0.1 µg/ml + Human U937 cell lysate (35µg protein in RIPA buffer).

    Predicted band size : 18 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab53536 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53536, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab53536 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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