Anti-Flagellin 抗体 (ab93713)
Key features and details
- Rabbit polyclonal to Flagellin
- Suitable for: WB, ICC/IF
- Reacts with: Escherichia coli, Salmonella anatum, Salmonella selandia, Salmonella
- Isotype: IgG
製品の概要
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製品名
Anti-Flagellin antibody
Flagellin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Flagellin -
由来種
Rabbit -
特異性
This antibody is specific for bacterial Flagellin (FliC). -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Escherichia coli, Salmonella anatum, Salmonella selandia, Salmonella -
免疫原
Purified recombinant Flagellin made in BL21 bacteria.
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ポジティブ・コントロール
- Lysates transfected with purified flagellin, E. Coli total lysate or 293T transfected with recombinant protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93713の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/10000 - 1/20000. Detects a band of approximately 60 kDa.
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ICC/IF |
1/100.
|
特記事項 |
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WB
1/10000 - 1/20000. Detects a band of approximately 60 kDa. |
ICC/IF
1/100. |
ターゲット情報
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関連性
Flagellin (FliC) is a subunit protein that polymerizes (along with other proteins) to form the filaments of bacterial flagella. Assembly of the bacterial flagellum occurs in a precise, temporal order where the basal component (FlgE, FlgK, and FlgL are assembled inside the bacterial membrane, followed by exportation of the filament cap protein FliD, and secretion of about 20,000 flagellin monomers (FliC) through the channel. FliC monomers are polymerized to form the tail filament. FliC monomers can function as pathogen-associated molecular patterns (PAMPs), and can be detected by host cells through surface-localized toll-like receptor 5 (TLR5) and cytosolic Nod-like receptors (NLRs). -
細胞内局在
Secreted. Bacterial flagellum. -
参照データベース
- Entrez Gene: 949101 Escherichia coli
- Entrez Gene: 961921 Escherichia coli
- SwissProt: P04949 Escherichia coli
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別名
- 41 kDa antigen antibody
- b1923 antibody
- flaF antibody
see all
画像
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All lanes : Anti-Flagellin antibody (ab93713) at 1/10000 dilution
All lanes : 293T transfected with recombinant protein
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.
Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI. -
Ab93713 (1:100) staining Flagellin in bone marrow-derived macrophages infected with salmonella.
Flagellin primary antiserum followed by Alexa488 secondary (green). Actin (red) stained with AlexaFluor 594-phalloidin. Nuclei stained with DAPI.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab93713 は 27 報の論文で使用されています。
- Gao F et al. Calreticulin (CALR)-induced activation of NF-ĸB signaling pathway boosts lung cancer cell proliferation. Bioengineered 13:6856-6865 (2022). PubMed: 35264066
- Liu T et al. The Rcs System Contributes to the Motility Defects of the Twin-Arginine Translocation System Mutant of Extraintestinal Pathogenic Escherichia coli. J Bacteriol 204:e0061221 (2022). PubMed: 35311558
- Cohen H et al. Intracellular Salmonella Paratyphi A is motile and differs in the expression of flagella-chemotaxis, SPI-1 and carbon utilization pathways in comparison to intracellular S. Typhimurium. PLoS Pathog 18:e1010425 (2022). PubMed: 35381053
- Northrop-Albrecht EJ et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
- Li F et al. Patatin-like phospholipase CapV in Escherichia coli - morphological and physiological effects of one amino acid substitution. NPJ Biofilms Microbiomes 8:39 (2022). PubMed: 35546554