製品の概要

  • 製品名Anti-FKBP52 antibody [EPR6619]
    FKBP52 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6619] to FKBP52
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cytmore details
    適用なし: ICC
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of Human FKBP52.

  • ポジティブ・コントロール
    • HeLa, MCF7, Jurkat and K562 cell lysates; Human breast tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

アプリケーション

Our Abpromise guarantee covers the use of ab124906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/200000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC.
  • ターゲット情報

    • 機能Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
    • 組織特異性Widely expressed.
    • 配列類似性Contains 2 PPIase FKBP-type domains.
      Contains 3 TPR repeats.
    • ドメインThe PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
      The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
      The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
    • 翻訳後修飾Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 51 kDa FK506-binding protein antibody
      • 52 kDa FK506 binding protein antibody
      • 52 kDa FK506-binding protein antibody
      • 52 kDa FKBP antibody
      • 59 kDa immunophilin antibody
      • FK506 binding protein 4 antibody
      • FK506-binding protein 4 antibody
      • FKBP 4 antibody
      • FKBP 52 antibody
      • FKBP 59 antibody
      • FKBP-4 antibody
      • FKBP-52 antibody
      • FKBP4 antibody
      • FKBP4_HUMAN antibody
      • FKBP51 antibody
      • FKBP52 protein antibody
      • FKBP59 antibody
      • HBI antibody
      • Hsp 56 antibody
      • HSP binding immunophilin antibody
      • HSP-binding immunophilin antibody
      • Hsp56 antibody
      • Immunophilin FKBP52 antibody
      • N-terminally processed antibody
      • p52 antibody
      • p59 antibody
      • p59 protein antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP4 antibody
      • Peptidylprolyl cis trans isomerase antibody
      • PPIase antibody
      • PPIase FKBP4 antibody
      • Rotamase antibody
      • T cell FK506 binding protein 59kD antibody
      see all

    Anti-FKBP52 antibody [EPR6619] 画像

    • All lanes : Anti-FKBP52 antibody [EPR6619] (ab124906) at 1/50000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
    • ab124906, at 1/100 dilution, staining FKBP52 in paraffin-embedded Human breast tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-FKBP52 antibody [EPR6619] (ab124906) 使用論文

    ab124906 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"