Anti-FKBP52 抗体 (ab97306)
Key features and details
- Rabbit polyclonal to FKBP52
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-FKBP52 antibody
FKBP52 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FKBP52 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to a region within amino acids 1-177 of Human FKBP52 (AAH01786)
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cells. Cal27 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97306の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. -
組織特異性
Widely expressed. -
配列類似性
Contains 2 PPIase FKBP-type domains.
Contains 3 TPR repeats. -
ドメイン
The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400. -
翻訳後修飾
Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 508535 Cow
- Entrez Gene: 2288 Human
- Entrez Gene: 14228 Mouse
- Entrez Gene: 260321 Rat
- Omim: 600611 Human
- SwissProt: Q9TRY0 Cow
- SwissProt: Q02790 Human
- SwissProt: P30416 Mouse
see all -
別名
- 51 kDa FK506-binding protein antibody
- 52 kDa FK506 binding protein antibody
- 52 kDa FK506-binding protein antibody
see all
画像
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Anti-FKBP52 antibody (ab97306) at 1/5000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS-PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab97306 at 1/200 dilution (1) and merged with DNA probe (2).
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab97306 at 1/500 dilution.
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Anti-FKBP52 antibody (ab97306) at 1/3000 dilution + Recombinant human FKBP52 protein (ab84189) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab97306 は 3 報の論文で使用されています。
- Chen X et al. Expression of genes involved in progesterone receptor paracrine signaling and their effect on litter size in pigs. J Anim Sci Biotechnol 7:31 (2016). WB, IHC . PubMed: 27231548
- Pare JM et al. Hsp90 cochaperones p23 and FKBP4 physically interact with hAgo2 and activate RNA interference-mediated silencing in mammalian cells. Mol Biol Cell 24:2303-10 (2013). PubMed: 23741051
- Martins RP et al. Proteomic analysis of porcine mesenteric lymph-nodes after Salmonella typhimurium infection. J Proteomics 75:4457-70 (2012). WB ; Pig . PubMed: 22507201