製品の概要

  • 製品名
    Anti-FKBP25 antibody [2364C3]
    FKBP25 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2364C3] to FKBP25
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment (Human)

  • ポジティブ・コントロール
    • HeLa whole cell lysate, HeLa cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    ab50750 was purified from from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
  • ポリ/モノ
    モノクローナル
  • クローン名
    2364C3
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab50750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/5.
WB 1/200. Predicted molecular weight: 25 kDa.

ターゲット情報

  • 機能
    FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. PPIases accelerate the folding of proteins.
  • 配列類似性
    Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 25 kDa FK506-binding protein antibody
    • 25 kDa FKBP antibody
    • FK506 binding protein 25 T cell antibody
    • FK506 binding protein 25, T cell, 25-KD antibody
    • FK506 binding protein 3 (25kD) antibody
    • FK506-binding protein 3 antibody
    • FKBP 25 antibody
    • FKBP 3 antibody
    • FKBP-25 antibody
    • FKBP-3 antibody
    • FKBP25 antibody
    • FKBP3 antibody
    • FKBP3_HUMAN antibody
    • Immunophilin FKBP25 antibody
    • Peptidyl-prolyl cis-trans isomerase FKBP3 antibody
    • PPIase antibody
    • PPIase FKBP3 antibody
    • Rapamycin binding protein antibody
    • Rapamycin-selective 25 kDa immunophilin antibody
    • Rotamase antibody
    see all

画像

  • Anti-FKBP25 antibody [2364C3] (ab50750) at 1/200 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse anti-IgG at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)
  • Ab50750 at 1/5 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution.

参考文献

ab50750 has not yet been referenced specifically in any publications.

レビューと Q&A

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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