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Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa960 and aa1020 of human FKBP135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).
Our Abpromise guarantee covers the use of ab14433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Detection of Human FKBP-135 by Western Blot and Immunoprecipitation. Samples: Nuclear extract (5 and 20 mcg for WB; 1 mg for IP) from HeLa cells. Antibodies: ab14432 used at 0.2 mcg/ml for WB in lane 1,2,3,4,5 and at 2 µg/mg extract for IP in lane 3 (B), ab14433 used at 2 µg/mg extract for IP in lane 4 (B) and ab14434 used at 2 mcg/mg extract for IP in lane 5 (B). Detection: chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).
ab14433 has not yet been referenced specifically in any publications.