Anti-FKBP135 抗体 (ab14433)

製品の概要

  • 製品名Anti-FKBP135 antibody
    FKBP135 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FKBP135
  • 特異性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: IHC-Fr, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    Synthetic peptide (Unfortunately the amino acid sequence is considered to be commercially sensitive). The peptide sequence is within the region between aa960 and aa1020 of human FKBP135 using the numbering given in TrEMBL entry Q9Y4D0 (GeneID 23307).

  • ポジティブ・コントロール
    • HeLa cells.
  • 特記事項Centrifuge tube to remove product from lid.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: Tris-citrate/phosphate. pH 7-8.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibody was purified using an epitope specific to FKBP135 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
WB
IP
IHC-P
  • 追加情報IHC-P: 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: Use at a concentration of 1 to 4 mcg/mg of lysate.
    WB: 1/2000 - 1/10000. Predicted molecular weight: 148 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
    Prepare working dilution immediately before use.
  • ターゲット情報

    • 機能May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.
    • 配列類似性Belongs to the FKBP-type PPIase family.
      Contains 1 PPIase FKBP-type domain.
    • ドメインThe PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
      The central coiled-coil region is responsible for association with early endosomes.
    • 細胞内局在Cytoplasm. Cell projection > axon. Early endosome. Present in axons and neuronal growth cones.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 133 kDa FK506-binding protein antibody
      • 133 kDa FKBP antibody
      • C430014M02Rik antibody
      • FK506 binding protein 135 antibody
      • FK506-binding protein 15 antibody
      • FKB15_HUMAN antibody
      • FKBO133 antibody
      • FKBP-133 antibody
      • FKBP-15 antibody
      • FKBP133 antibody
      • Fkbp15 antibody
      • KIAA0674 antibody
      • mKIAA0674 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • RIKEN cDNA C430014M02 gene antibody
      • RP23 64F17.7 antibody
      • WAFL antibody
      • WASP and FKBP-like antibody
      see all

    Anti-FKBP135 antibody 画像

    • Detection of Human FKBP-135 by Western Blot and Immunoprecipitation. Samples: Nuclear extract (5 and 20 mcg for WB; 1 mg for IP) from HeLa cells. Antibodies: ab14432 used at 0.2 mcg/ml for WB in lane 1,2,3,4,5 and at 2 µg/mg extract for IP in lane 3 (B), ab14433 used at 2 µg/mg extract for IP in lane 4 (B) and ab14434 used at 2 mcg/mg extract for IP in lane 5 (B). Detection: chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).

    Anti-FKBP135 antibody (ab14433) 使用論文

    ab14433 has not yet been referenced specifically in any publications.

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    I am sorry if it was misleading but the image supplied with Anti-FKBP135 antibodies ab14432, ab14433 and ab14434 are the same. They illustrate the IP performed with ab14432,...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Liver)
    Specification Liver
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 1X
    Permeabilization No
    Blocking step Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    投稿 Jan 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Penile Tissue)
    Specification Penile Tissue
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.4% Triton/PBS
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15µg/mL · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    投稿 Dec 10 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Penile Tissue Sections)
    Specification Penile Tissue Sections
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer 1X
    Permeabilization Yes - TBST 15 minutes
    Blocking step Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    投稿 Dec 10 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Rat Tissue lysate - whole (Penile Tissue)
    Loading amount 60 µg
    Specification Penile Tissue
    Gel Running Conditions Reduced Denaturing
    Blocking step Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. gwen Lagoda

    Verified customer

    投稿 Sep 18 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"