製品の概要

  • 製品名Anti-FKBP135 antibody
    FKBP135 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FKBP135
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Horse, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human FKBP135 aa 50-100.

  • ポジティブ・コントロール
    • HeLa cells nuclear extract.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Antibody was purified using an epitope specific to FKBP-135 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF 1/200.
WB 1/2000 - 1/10000. Predicted molecular weight: 135 kDa.
IP Use a concentration of 1 - 4 mg/ml.

ターゲット情報

  • 機能May be involved in the cytoskeletal organization of neuronal growth cones. Seems to be inactive as a PPIase (By similarity). Involved in the transport of early endosomes at the level of transition between microfilament-based and microtubule-based movement.
  • 配列類似性Belongs to the FKBP-type PPIase family.
    Contains 1 PPIase FKBP-type domain.
  • ドメインThe PPIase FKBP-type domain seems to be inactive both for FK506-binding and enzymatic activity.
    The central coiled-coil region is responsible for association with early endosomes.
  • 細胞内局在Cytoplasm. Cell projection > axon. Early endosome. Present in axons and neuronal growth cones.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 133 kDa FK506-binding protein antibody
    • 133 kDa FKBP antibody
    • C430014M02Rik antibody
    • FK506 binding protein 135 antibody
    • FK506-binding protein 15 antibody
    • FKB15_HUMAN antibody
    • FKBO133 antibody
    • FKBP-133 antibody
    • FKBP-15 antibody
    • FKBP133 antibody
    • Fkbp15 antibody
    • KIAA0674 antibody
    • mKIAA0674 antibody
    • Peptidyl prolyl cis trans isomerase antibody
    • RIKEN cDNA C430014M02 gene antibody
    • RP23 64F17.7 antibody
    • WAFL antibody
    • WASP and FKBP-like antibody
    see all

Anti-FKBP135 antibody 画像

  • ab14432 staining FKBP135 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. An Alexa Fluor®597-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Cells labelled for FKBP15 displayed punctate staining, which was absent in cells treated with siRNA against FKBP15.

    See Abreview

  • All lanes : Anti-FKBP135 antibody (ab14432) at 0.2 µg/ml

    Lane 1 : Nuclear lysates prepared from Hela cells at 5 µg
    Lane 2 : Nuclear lysates prepared from Hela cells at 20 µg
    Lane 3 : Nuclear lysates prepared from Hela cells at 1 mg/ml
    Lane 4 : Nuclear lysates prepared from Hela cells at 1 mg/ml
    Lane 5 : Nuclear lysates prepared from Hela cells at 1 mg/ml


    Predicted band size : 135 kDa

    Antibodies: ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection: Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).

    In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2µg/mg of extract.

  • ab14432 at 1/1000 staining mouse liver tissue sections by IHC-P. The tissue sections were paraformaldehyde fixed and blocked prior to incubation with the ab14432 for 24 hours. An HRP conjugated secondary antibody was used. The image shows staining in liver cells of a female mouse at 20X magnification.

    See Abreview

Anti-FKBP135 antibody (ab14432) 使用論文

This product has been referenced in:
  • Zavodszky E  et al. Mutation in VPS35 associated with Parkinson's disease impairs WASH complex association and inhibits autophagy. Nat Commun 5:3828 (2014). ICC/IF ; Human . Read more (PubMed: 24819384) »
  • Viklund IM  et al. Identification of a new WASP and FKBP-like (WAFL) protein in inflammatory bowel disease: a potential marker gene for ulcerative colitis. Int J Colorectal Dis 23:921-30 (2008). IHC (PFA fixed) ; Human . Read more (PubMed: 18654788) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.1% Triton TX-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 May 23 2013

Thank you for contacting us and your interest in our products.


I am sorry if it was misleading but the image supplied with Anti-FKBP135 antibodies ab14432, ab14433 and ab14434 are the same. They illustrate the IP performed with ab14432,...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Miss. gwen Lagoda

Verified customer

投稿 Apr 27 2007

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Penis Sections)
Specification Penis Sections
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100%
Username

Miss. gwen Lagoda

Verified customer

投稿 Apr 12 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Human Penis)
Specification Human Penis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Blocking step Dako Peroxidase Blocking Reagent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100
Username

Miss. gwen Lagoda

Verified customer

投稿 Feb 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"