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RabMAb

Anti-FKBP12 抗体 [EPR3888] (ab92459)

製品の概要

  • 製品名
    Anti-FKBP12 antibody [EPR3888]
    FKBP12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3888] to FKBP12
  • アプリケーション
    適用あり: WB, ICCmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues at the C terminus of Human FKBP12.

  • ポジティブ・コントロール
    • Fetal brain, U937 and SH-SY5Y cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR3888
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab92459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC
  • 追加情報
    ICC: 1/50 - 1/100.
    WB: 1/10,000 - 1/50,000. Predicted molecular weight: 12 kDa.

    Is unsuitable for Flow Cyt, IHC-P or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
    • 配列類似性
      Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
      Contains 1 PPIase FKBP-type domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 12 kDa FK506-binding protein antibody
      • 12 kDa FKBP antibody
      • Calstabin 1 antibody
      • FK506 binding protein 1 antibody
      • FK506 binding protein 12 antibody
      • FK506 binding protein 1A (12kD) antibody
      • FK506 binding protein 1A 12kDa antibody
      • FK506 binding protein 1A antibody
      • FK506 binding protein T cell 12 kD antibody
      • FK506 binding protein, T cell, 12 kD antibody
      • FK506 binding protein12 antibody
      • FK506-binding protein 1A antibody
      • FKB1A_HUMAN antibody
      • FKBP 12 antibody
      • FKBP 1A antibody
      • FKBP-12 antibody
      • FKBP-1A antibody
      • FKBP1 antibody
      • FKBP12 antibody
      • FKBP12 Exip3 antibody
      • FKBP12C antibody
      • fkbp1a antibody
      • Immunophilin FKBP12 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
      • PKC12 antibody
      • PKCI2 antibody
      • PPIase antibody
      • PPIase FKBP1A antibody
      • Protein kinase C inhibitor 2 antibody
      • Rotamase antibody
      see all

    画像

    • All lanes : Anti-FKBP12 antibody [EPR3888] (ab92459) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : U937 cell lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 12 kDa

    参考文献

    ab92459 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (cultured cell)
    Gel Running Conditions
    Reduced Denaturing (4%-12% bis tris)
    Loading amount
    30 µg
    Specification
    cultured cell
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Mar 09 2016

    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (Exponential growth)
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Loading amount
    10 µg
    Specification
    Exponential growth
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 09 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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