製品の概要

  • 製品名
    Anti-FKBP12 antibody [EP3887]
    FKBP12 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP3887] to FKBP12
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human FKBP12 aa 1-100 (internal sequence). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HL-60, U937 and SH-SY5Y lysates; HeLa cells
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP3887
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108420 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
ICC/IF 1/50 - 1/100.
  • 追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能
      May play a role in modulation of ryanodine receptor isoform-1 (RYR-1), a component of the calcium release channel of skeletal muscle sarcoplasmic reticulum. There are four molecules of FKBP12 per skeletal muscle RYR. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
    • 配列類似性
      Belongs to the FKBP-type PPIase family. FKBP1 subfamily.
      Contains 1 PPIase FKBP-type domain.
    • 細胞内局在
      Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 12 kDa FK506-binding protein antibody
      • 12 kDa FKBP antibody
      • Calstabin 1 antibody
      • FK506 binding protein 1 antibody
      • FK506 binding protein 12 antibody
      • FK506 binding protein 1A (12kD) antibody
      • FK506 binding protein 1A 12kDa antibody
      • FK506 binding protein 1A antibody
      • FK506 binding protein T cell 12 kD antibody
      • FK506 binding protein, T cell, 12 kD antibody
      • FK506 binding protein12 antibody
      • FK506-binding protein 1A antibody
      • FKB1A_HUMAN antibody
      • FKBP 12 antibody
      • FKBP 1A antibody
      • FKBP-12 antibody
      • FKBP-1A antibody
      • FKBP1 antibody
      • FKBP12 antibody
      • FKBP12 Exip3 antibody
      • FKBP12C antibody
      • fkbp1a antibody
      • Immunophilin FKBP12 antibody
      • Peptidyl prolyl cis trans isomerase antibody
      • Peptidyl-prolyl cis-trans isomerase FKBP1A antibody
      • PKC12 antibody
      • PKCI2 antibody
      • PPIase antibody
      • PPIase FKBP1A antibody
      • Protein kinase C inhibitor 2 antibody
      • Rotamase antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: FKBP12 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: SH-SY5Y whole cell lysate (20 µg)
      Lane 4: Human brain whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108420 observed at 12 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab108420 was shown to recognize FKBP12 in wild-type HAP1 cells as signal was lost at the expected MW in FKBP12 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FKBP12 knockout samples were subjected to SDS-PAGE. Ab108420 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-FKBP12 antibody [EP3887] (ab108420) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : HL-60 brain lysate
      Lane 3 : U937 lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 12 kDa

    • Immunofluorescent staining of FKBP12 in HeLa cells using ab108420 at 1/50 dilution.

    参考文献

    ab108420 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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