製品の概要

  • 製品名Anti-FISH antibody
    FISH 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to FISH
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    C-KPIEKSQFIHNNLK

    , corresponding to amino acids 1033-1046 of Human FISH (NP_055446.2).

  • ポジティブ・コントロール
    • Human Liver, and Small Intestine tissues

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3.75 - 7.5 µg/ml.

ターゲット情報

  • 機能Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of beta-amyloid peptide.
  • 組織特異性Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.
  • 配列類似性Belongs to the SH3PXD2 family.
    Contains 1 PX (phox homology) domain.
    Contains 5 SH3 domains.
  • ドメインThe PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,4-biphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-biphosphate (PtdIns(5)P), and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain.
    The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19.
  • 翻訳後修飾Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Cytoplasm. Cell projection > podosome. Cytoplasmic in normal cells and localizes to podosomes in SRC-transformed cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adapter protein TKS5 antibody
    • Five SH3 domain-containing protein antibody
    • SH3 and PX domain-containing protein 2A antibody
    • SH3 multiple domains protein 1 antibody
    • Sh3md1 antibody
    • Sh3pxd2a antibody
    • SPD2A_HUMAN antibody
    • TKs5 antibody
    • Tyrosine kinase substrate with five SH3 domains antibody
    see all

Anti-FISH antibody 画像

  • Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab118575 at a concentration of 7.5 µg/ml.
  • Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE) using ab118575 at a concentration of 7.5 µg/ml.

Anti-FISH antibody (ab118575) 使用論文

This product has been referenced in:
  • Gill DJ  et al. Initiation of GalNAc-type O-glycosylation in the endoplasmic reticulum promotes cancer cell invasiveness. Proc Natl Acad Sci U S A 110:E3152-61 (2013). Read more (PubMed: 23912186) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"