Anti-Firefly Luciferase 抗体 [mAb21] (ab64564)


  • 製品名Anti-Firefly Luciferase antibody [mAb21]
    Firefly Luciferase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAb21] to Firefly Luciferase
  • 特異性The clone number has been updated from (3i1) to (mAb21) both clone numbers name the same antibody clone.
  • アプリケーション適用あり: WB, ELISA, ICCmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Firefly
  • 免疫原

    Full-length recombinant luciferase from Photinus pyralis.

  • ポジティブ・コントロール
    • 3T3 cell lysate containing 5ng of recombinant luciferase


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 0.1M Tris glycine, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名mAb21
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab64564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    Is unsuitable for IHC-P.
    WB: Use at a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 66 kDa.
    Note: 0.5-2 µg/ml detects 5ng of recombinant luciferase that was added to 3T3 cell lysate.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
    • 細胞内局在Peroxisome
    • 参照データベース
    • 別名
      • ec antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    Anti-Firefly Luciferase antibody [mAb21] 画像

    • All lanes : Anti-Firefly Luciferase antibody [mAb21] (ab64564) at 5 µg/ml

      Lane 1 : Non-transfected 293 whole cell lysate
      Lane 2 : Firefly Luciferase transfected 293 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : NIH3T3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 66 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

      Lanes 1 - 4: Merged signal (red and green). Green – ab64564 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab64564 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    Anti-Firefly Luciferase antibody [mAb21] (ab64564) 使用論文

    ab64564 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 0.05 µg
    Gel Running Conditions Reduced Denaturing (4-15% Tris-glycine)
    Sample Firefly (Photinus pyralis) Recombinant protein (QuantiLum Recombinant Luciferase (Promega))
    Specification QuantiLum Recombinant Luciferase (Promega)
    Treatment Heated in the presence of sHSP followed by UV crosslinking
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

    Miss. Rachel Loh

    Verified customer

    投稿 Jan 13 2015

    We have no firefly luciferase antibody tested in IP which is mouse or rabbit origin. Only ab498- Goat polyclonal to firefly Luciferase Other Firefly Luciferase antibodies tested in WB: 1. ab16466 Firefly Luciferase antibody [Luci17] Mouse monocl...

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