Anti-Firefly Luciferase 抗体 [mAb21] (ab64564)

製品の概要

  • 製品名
    Anti-Firefly Luciferase antibody [mAb21]
    Firefly Luciferase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAb21] to Firefly Luciferase
  • 特異性
    The clone number has been updated from (3i1) to (mAb21) both clone numbers name the same antibody clone.
  • アプリケーション
    適用あり: WB, ELISA, ICCmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Firefly
  • 免疫原

    Full-length recombinant luciferase from Photinus pyralis.

  • ポジティブ・コントロール
    • 3T3 cell lysate containing 5ng of recombinant luciferase
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab185923 as a replacement.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 0.1M Tris glycine, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    mAb21
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
ICC
  • 追加情報
    ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    Is unsuitable for IHC-P.
    WB: Use at a concentration of 0.2 - 5 µg/ml. Predicted molecular weight: 66 kDa.
    Note: 0.5-2 µg/ml detects 5ng of recombinant luciferase that was added to 3T3 cell lysate.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
    • 細胞内局在
      Peroxisome
    • 参照データベース
    • 別名
      • ec 1.13.12.7 antibody
      • Firefly antibody
      • Luciferase antibody
      • Luciferin 4 monooxygenase antibody

    画像

    • All lanes : Anti-Firefly Luciferase antibody [mAb21] (ab64564) at 5 µg/ml

      Lane 1 : Non-transfected 293 whole cell lysate
      Lane 2 : Firefly Luciferase transfected 293 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : NIH3T3 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 66 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

      Lanes 1 - 4: Merged signal (red and green). Green – ab64564 observed at 65 kDa. Red - loading control, ab181602, observed at 37 kDa.

       

      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab64564 and ab181602 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at a 1:10000 dilution for 1hr at room temperature and then imaged.

    参考文献

    ab64564 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Loading amount
    0.05 µg
    Gel Running Conditions
    Reduced Denaturing (4-15% Tris-glycine)
    Sample
    Firefly (Photinus pyralis) Recombinant protein (QuantiLum Recombinant Luciferase (Promega))
    Specification
    QuantiLum Recombinant Luciferase (Promega)
    Treatment
    Heated in the presence of sHSP followed by UV crosslinking
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Miss. Rachel Loh

    Verified customer

    投稿 Jan 13 2015

    We have no firefly luciferase antibody tested in IP which is mouse or rabbit origin. Only ab498- Goat polyclonal to firefly Luciferase Other Firefly Luciferase antibodies tested in WB: 1. ab16466 Firefly Luciferase antibody [Luci17] Mouse monocl...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録