Recombinant fragment corresponding to Human Filamin B aa 30-239.
Database link: O75369
Our Abpromise guarantee covers the use of ab97457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/500 - 1/3000. Predicted molecular weight: 278 kDa. | |
ICC/IF | 1/100 - 1/200. |
5% SDS PAGE.
Secondary antibody - goat anti-rabbit HRP (ab6721)
5% SDS PAGE.
Secondary antibody - goat anti-rabbit HRP (ab6721)
ab97547, at a 1/200 dilution, staining Filamin B in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.
Immunofluorescence analysis of Human trabecular meshwork cells, staining Filamin B with ab97457.
Left panel: Filamin B staining alone. Right panel: Filamin B staining (red) merged with F-actin staining (green).
Cells were treated with dexamethasone, before fixing in paraformaldehyde and permeabilizing wih 0.2% Triton X-100. Cells were incubated with primary antibody (10 µg/ml) and AlexaFluor®488-conjugated phalloidin. Filamin B staining was detected using an AlexaFluor®546-conjugated goat anti-rabbit IgG.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"