Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) 抗体 [EP2310AY] (ab75978)

製品の概要

  • 製品名Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY]
  • 製品の詳細
    Rabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107)
  • 特異性ab75978 detects Filamin A phosphorylated on serine 2152 and Filamin B phosphorylated on serine 2107.
  • アプリケーション適用あり: ICC/IF, ELISA, WB, IHC-Pmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Filamin A (phospho S2152) + Filamin B (phospho S2107) aa 2100-2200.
    Database link: P21333

  • ポジティブ・コントロール
    • HeLa cell lysates, untreated or treated with calyculin A; human uterus tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/200.
ELISA Use at an assay dependent concentration. PubMed: 21507248
WB 1/10000 - 1/20000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 関連性Filamin A-P21333. Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Filamin B-O75369. Function: Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.
    • 参照データベース
    • 別名
      • ABP-278 antibody
      • ABP-280 antibody
      • ABP-280 homolog antibody
      • Actin-binding protein 280 antibody
      • Actin-binding-like protein antibody
      • Alpha-filamin antibody
      • Beta-filamin antibody
      • Endothelial actin-binding protein antibody
      • Fh1 antibody
      • Filamin homolog 1 antibody
      • Filamin-1 antibody
      • Filamin-3 antibody
      • Filamin-A antibody
      • Filamin-B antibody
      • FLN antibody
      • FLN-A antibody
      • FLN1 antibody
      • FLN1L antibody
      • FLN3 antibody
      • FLNA antibody
      • FLNB antibody
      • Non-muscle filamin antibody
      • TABP antibody
      • TAP antibody
      • Thyroid autoantigen antibody
      • Truncated ABP antibody
      • Truncated actin-binding protein antibody
      see all

    Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] 画像

    • All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/20000 dilution

      Lane 1 : HeLa lysate, untreated
      Lane 2 : HeLa lysate, treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 281 kDa
      Observed band size : 281 kDa
    • Immunofluorescence analysis of U. parvum infected BPH-1 (Human benign prostate) cells staining Filamin A (phospho S2152) and Filamin B (phospho S2107) with ab75978 (red). Cells were fixed with formaldehyde before incubating with primary antibody. An AlexaFluor®594-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    • ab75978 at 1/100 dilution staining Filamin A (phospho S2152) and Filamin B (phospho S2107) in human uterus by Immunohistochemistry, Paraffin-embedded tissue.

    Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) 使用論文

    This product has been referenced in:
    • Allam AB  et al. Ureaplasma parvum infection alters filamin A dynamics in host cells. BMC Infect Dis 11:101 (2011). ICC/IF, ELISA ; Rat . Read more (PubMed: 21507248) »
    • Bogatcheva NV  et al. Molecular mechanisms mediating protective effect of cAMP on lipopolysaccharide (LPS)-induced human lung microvascular endothelial cells (HLMVEC) hyperpermeability. J Cell Physiol 221:750-9 (2009). WB ; Human . Read more (PubMed: 19725051) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your recent telephone enquiry and for your patience. It has taken some time to obtain the immunogen information for you.

    For the two rabbit monoclonal antibodies:

    ab76289
    Immunogen: from within amino acids range...

    Read More

    Thank you for your inquiry. HeLa cells are serum-starved overnight, and then treated with 100 nM Calyculin A for 30 min at 37 deg C. Calyculin A was chosen because it is a known serine/threonine phosphatase inhibitor. 1.Resjö, S. et al. ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"