Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) 抗体 [EP2310AY] (ab75978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY]
Filamin A (phospho S2152) + Filamin B (phospho S2107) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2310AY] to Filamin A (phospho S2152) + Filamin B (phospho S2107) -
由来種
Rabbit -
特異性
ab75978 detects Filamin A phosphorylated on serine 2152 and Filamin B phosphorylated on serine 2107.
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アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HEK-293. NIH/3T3, and C6 whole cell lysates. IHC-P: Human, Mouse, and Rat kidney tissue.
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特記事項
This product has switched from a hybridoma to recombinant production method on 9th June 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2310AY -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75978の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/100000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
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IHC-P |
1/1200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/100000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa). |
IHC-P
1/1200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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関連性
Filamin A-P21333. Function: Promotes orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton and serves as a scaffold for a wide range of cytoplasmic signaling proteins. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Tethers cell surface-localized furin, modulates its rate of internalization and directs its intracellular trafficking. Involved in ciliogenesis. Filamin B-O75369. Function: Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro. -
参照データベース
- Entrez Gene: 2316 Human
- Entrez Gene: 2317 Human
- Entrez Gene: 192176 Mouse
- Entrez Gene: 286940 Mouse
- Entrez Gene: 293860 Rat
- Entrez Gene: 306204 Rat
- SwissProt: O75369 Human
- SwissProt: P21333 Human
see all -
別名
- ABP-278 antibody
- ABP-280 antibody
- ABP-280 homolog antibody
see all
画像
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All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/100000 dilution
Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate
Lane 3 : HEK-293 (Human embryonic kidney epithelial cell) treated with 50nM Calyculin A for 3 hours whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 281 kDa
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/100000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 : Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 281 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Filamin A (phospho S2152) + Filamin B (phospho S2107) antibody [EP2310AY] (ab75978) at 1/100000 dilution
Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane was incubated with Alkaline phosphatase
Lysates/proteins at 15 µg per lane.
Predicted band size: 281 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Human kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Mouse kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling Filamin A (phospho S2152) + Filamin B (phospho S2107) with ab75978 at 1/1200 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on Rat kidney tissue without alkaline phosphatase treatment (A) compared to no signal detected when treated with alkaline phosphatase (B). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval Solution2) for 30 mins. Counterstained with hematoxylin.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab75978 は 2 報の論文で使用されています。
- Allam AB et al. Ureaplasma parvum infection alters filamin A dynamics in host cells. BMC Infect Dis 11:101 (2011). ICC/IF, ELISA ; Rat . PubMed: 21507248
- Bogatcheva NV et al. Molecular mechanisms mediating protective effect of cAMP on lipopolysaccharide (LPS)-induced human lung microvascular endothelial cells (HLMVEC) hyperpermeability. J Cell Physiol 221:750-9 (2009). WB ; Human . PubMed: 19725051