製品の概要

  • 製品名Anti-Filaggrin antibody
    Filaggrin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Filaggrin
  • 特異性ab81468 reacts specifically with the human 435 kDa protein.
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from the N terminal domain of human Filaggrin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200 - 1/1000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 435 kDa.
ICC/IF 1/1000.

ターゲット情報

  • 機能Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • 組織特異性Keratohyalin granules.
  • 関連疾患Ichthyosis vulgaris
    Dermatitis atopic 2
  • 配列類似性Belongs to the S100-fused protein family.
    Contains 2 EF-hand domains.
    Contains 23 filaggrin repeats.
  • 翻訳後修飾Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
    Undergoes deimination of some arginine residues (citrullination).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epidermal filaggrin antibody
    • FILA_HUMAN antibody
    • Filaggrin antibody
    • Filaggrin precursor antibody
    • Fillagrin antibody
    • FLG antibody
    • Profilaggrin antibody
    see all

Anti-Filaggrin antibody 画像

  • ICC/IF image of ab81468 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81468, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Filaggrin antibody (ab81468) 使用論文

This product has been referenced in:
  • Kiritsi D  et al. Targeting epidermal lipids for treatment of Mendelian disorders of cornification. Orphanet J Rare Dis 9:33 (2014). IHC-Fr ; Human . Read more (PubMed: 24607067) »
  • Dahlqvist J  et al. siRNA silencing of proteasome maturation protein (POMP) activates the unfolded protein response and constitutes a model for KLICK genodermatosis. PLoS One 7:e29471 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22235297) »

See all 2 Publications for this product

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Vielen Dank für Ihre Rückmeldung. Aus unseren Unterlagen geht hervor, dass der Antikörper im Western blot routinemäßig in einer Verdünnungsreihe getestet wurde, die wie auf dem Datenblatt angegeben Verdünnungen zwischen 1:500 und 1:5000 beinhaltete....

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Thank you for your email. I am very sorry that it took so long to get back to you. I have been in contact with the lab that produces this antibody and it was very difficult to get satisfactory information about ab81468. According to the informati...

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