製品の概要

  • 製品名Anti-Fibulin 5 antibody [EPR4506]
    Fibulin 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4506] to Fibulin 5
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant fragment corresponding to Human Fibulin 5 aa 250 to the C-terminus (internal sequence).

  • ポジティブ・コントロール
    • WB: Human fetal lung, fetal kidney, fetal spleen and testis tissue lysates. Mouse heart, brain and kidney tissue lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 50 kDa.

ターゲット情報

  • 機能Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling.
  • 組織特異性Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.
  • 関連疾患Defects in FBLN5 are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. Autosomal dominant cutis laxa is a relatively benign inherited and acquired connective tissue disorder.
    Defects in FBLN5 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
    Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3) [MIM:608895]. ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性Belongs to the fibulin family.
    Contains 6 EGF-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADCL2 antibody
    • ARCL1A antibody
    • ARMD3 antibody
    • Dance antibody
    • Developmental arteries and neural crest EGF like protein antibody
    • Developmental arteries and neural crest EGF-like protein antibody
    • EVEC antibody
    • Fbln5 antibody
    • FBLN5_HUMAN antibody
    • FIBL 5 antibody
    • FIBL-5 antibody
    • Fibulin-5 antibody
    • FLJ90059 antibody
    • UP50 antibody
    • Urine p50 protein antibody
    see all

Anti-Fibulin 5 antibody [EPR4506] 画像

  • All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50-60 kDa (why is the actual band size different from the predicted?)

    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50-60 kDa (why is the actual band size different from the predicted?)

    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Fibulin 5 antibody [EPR4506] (ab109428) at 1/1000 dilution (unpurified)

    Lane 1 : Fetal lung lysate
    Lane 2 : Fetal kidney lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 50 kDa

Anti-Fibulin 5 antibody [EPR4506] (ab109428) 使用論文

ab109428 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"