製品の概要

  • 製品名Anti-Fibulin 5 antibody [1G6A4]
    Fibulin 5 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1G6A4] to Fibulin 5
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant His tagged fragment, corresponding to amino acids 242-448 of Human Fibulin 5

  • ポジティブ・コントロール
    • Colon and breast cancer

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66339 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 50 kDa.
ELISA 1/10000.
IHC-P 1/500 - 1/2000.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Could be a vascular ligand for integrin receptors and may play a role in vascular development and remodeling.
  • 組織特異性Expressed predominantly in heart, ovary, and colon but also in kidney, pancreas, testis, lung and placenta. Not detectable in brain, liver, thymus, prostate, or peripheral blood leukocytes.
  • 関連疾患Defects in FBLN5 are a cause of autosomal dominant cutis laxa (ADCL) [MIM:123700]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. Autosomal dominant cutis laxa is a relatively benign inherited and acquired connective tissue disorder.
    Defects in FBLN5 are a cause of cutis laxa autosomal recessive type 1 (ARCL1) [MIM:219100]. Hereditary cutis laxa refers to a heterogeneous group of connective tissue disorders characterized by cutaneous abnormalities and variable systemic manifestations. The most constant clinical feature is loose skin, sagging over the face and trunk. Hereditary cutis laxa is inherited in both autosomal dominant and autosomal recessive modes. ARCL1 shows the most severe phenotype and has the poorest prognosis. In addition to the skin, internal organs enriched in elastic fibers, such as the lung and arteries, are affected.
    Defects in FBLN5 are the cause of age-related macular degeneration type 3 (ARMD3) [MIM:608895]. ARMD is a multifactorial disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid (known as drusen) that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
  • 配列類似性Belongs to the fibulin family.
    Contains 6 EGF-like domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADCL2 antibody
    • ARCL1A antibody
    • ARMD3 antibody
    • Dance antibody
    • Developmental arteries and neural crest EGF like protein antibody
    • Developmental arteries and neural crest EGF-like protein antibody
    • EVEC antibody
    • Fbln5 antibody
    • FBLN5_HUMAN antibody
    • FIBL 5 antibody
    • FIBL-5 antibody
    • Fibulin-5 antibody
    • FLJ90059 antibody
    • UP50 antibody
    • Urine p50 protein antibody
    see all

Anti-Fibulin 5 antibody [1G6A4] 画像

  • Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/2000 dilution + Human vascular smooth muscle whole cell lysate at 20 µg

    Secondary
    HRP-conjugated Rabbit anti-mouse IgG polyclonal at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-Fibulin 5 antibody [1G6A4] (ab66339) at 1/500 dilution + Immunogen (recombinant truncated protein) at 1 µg

    Predicted band size : 50 kDa
  • Immunohistochemical analysis of paraffin embedded human colon cancer tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue showing cytoplasmic localisation with DAB staining using ab66337 at 1/500 dilution.
  • ICC/IF image of ab66339 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66339, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Fibulin 5 antibody [1G6A4] (ab66339) 使用論文

This product has been referenced in:
  • Pérez-Rico C  et al. Elastin development-associated extracellular matrix constituents of subepithelial connective tissue in human pterygium. Invest Ophthalmol Vis Sci 55:6309-18 (2014). Human . Read more (PubMed: 25183766) »
  • Munjal C  et al. TGF-ß mediates early angiogenesis and latent fibrosis in an Emilin1-deficient mouse model of aortic valve disease. Dis Model Mech 7:987-96 (2014). IHC ; Mouse . Read more (PubMed: 25056700) »

See all 4 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10% polyacrylamide)
Sample Human Cell lysate - whole cell (human vascular smooth muscle cells)
Specification human vascular smooth muscle cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 04 2013

Thank you for contacting us, and I apologize that our packaging did not arrive intact. I am not sure if the cooling solution could have entered the antibody vial, and if it did I don't know what kind of effect that would have on the product quality. Ju...

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