Anti-Fibulin 1 抗体 [EPR10913] (ab175204)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10913] to Fibulin 1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Fibulin 1 antibody [EPR10913]
Fibulin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10913] to Fibulin 1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment within Human Fibulin 1 aa 250-450. The exact sequence is proprietary.
Database link: P23142 -
ポジティブ・コントロール
- Human placenta, fetal liver and fetal heart tissue lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10913 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175204の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 77 kDa. |
ターゲット情報
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機能
Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP. -
組織特異性
Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells. -
関連疾患
Note=A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
Note=Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer. -
配列類似性
Belongs to the fibulin family.
Contains 3 anaphylatoxin-like domains.
Contains 9 EGF-like domains. -
発生段階
Widely expressed during embryonic development. Prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. In embryos of gestational week (gw) 4, staining was observed in the early mesenchymal bone anlagen. In gw 6.5 and 8, all perichondrial structures showed expression but the chondrocytes themselves showed no staining. In gw 10, expression is prominent in the interterritorial matrix surrounding the hypertrophic chondrocytes. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 2192 Human
- Entrez Gene: 14114 Mouse
- Entrez Gene: 315191 Rat
- Omim: 135820 Human
- SwissProt: P23142 Human
- SwissProt: Q08879 Mouse
- Unigene: 24601 Human
- Unigene: 593123 Human
see all -
別名
- Basement membrane protein 90 antibody
- BM 90 antibody
- FBLN antibody
see all
画像
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All lanes : Anti-Fibulin 1 antibody [EPR10913] (ab175204) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Fetal liver tissue lysate
Lane 3 : Fetal heart tissue lysate
Lysates/proteins at 1/10 dilution per lane.
Predicted band size: 77 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab175204 は 4 報の論文で使用されています。
- Xu G et al. FBLN1 promotes chondrocyte proliferation by increasing phosphorylation of Smad2. J Orthop Sci N/A:N/A (2021). PubMed: 33610427
- Liu G et al. Fibulin-1c regulates transforming growth factor-ß activation in pulmonary tissue fibrosis. JCI Insight 5:N/A (2019). PubMed: 31343988
- Lertsuwan K et al. Identification of Fibulin-1 as a Human Bone Marrow Stromal (HS-5) Cell-Derived Factor That Induces Human Prostate Cancer Cell Death. Prostate 77:729-742 (2017). PubMed: 28168724
- Liu G et al. Fibulin-1 regulates the pathogenesis of tissue remodeling in respiratory diseases. JCI Insight 1:N/A (2016). PubMed: 27398409