製品の概要

  • 製品名Anti-Fibulin 1 antibody
    Fibulin 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Fibulin 1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: GDLDVGGLQE TDKIIEVEEE QEDPYLNDRC RGGGPCKQQC RDTGDEVVCS CFVGYQLLSD GVSCEDVNEC ITGSHSCRLG ESCINTVGSF RCQRDSSCGT , corresponding to amino acids 151-251 of Human Fibulin 1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 77 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.
  • 組織特異性Isoform A and isoform B are only expressed in placenta. Isoform C and isoform D are expressed in a variety of tissues and cultured cells.
  • 関連疾患Note=A chromosomal aberration involving FBLN1 is found in a complex type of synpolydactyly referred to as 3/3-prime/4 synpolydactyly associated with metacarpal and metatarsal synostoses. Reciprocal translocation t(12;22)(p11.2;q13.3) with C12orf2. Fibroblasts derived from a patient with synpolydactyly displayed alterations in the level of isoform D splice variant incorporated into the ECM and secreted into the conditioned culture medium. By contrast, the expression of isoform C was not perturbed in the patients fibroblasts. Furthermore, no aberrant polypeptides were detected in extracts of cultured patients fibroblasts. The translocation t(12;22) may result in haploinsufficiency of the isoform D splice variant, which could lead to the observed limb malformation.
    Note=Elevated expression and altered processing of FBLN1 protein is associated with human breast cancer.
  • 配列類似性Belongs to the fibulin family.
    Contains 3 anaphylatoxin-like domains.
    Contains 9 EGF-like domains.
  • 発生段階Widely expressed during embryonic development. Prominent in the matrix of the leptomeningeal anlage, in basement membranes of the neuroepithelium and the perineurium of peripheral nerves. In embryos of gestational week (gw) 4, staining was observed in the early mesenchymal bone anlagen. In gw 6.5 and 8, all perichondrial structures showed expression but the chondrocytes themselves showed no staining. In gw 10, expression is prominent in the interterritorial matrix surrounding the hypertrophic chondrocytes.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basement membrane protein 90 antibody
    • BM 90 antibody
    • FBLN antibody
    • Fbln1 antibody
    • FBLN1_HUMAN antibody
    • FIBL 1 antibody
    • FIBL-1 antibody
    • FIBL1 antibody
    • Fibulin 1 antibody
    • Fibulin-1 antibody
    see all

Anti-Fibulin 1 antibody 画像



  • Predicted band size : 77 kDa
    Fibulin 1 antibody (ab54652) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ICC/IF image of ab54652 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54652, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Fibulin 1 antibody (ab54652) 使用論文

This product has been referenced in:
  • Kotula E  et al. DNA-PKcs plays role in cancer metastasis through regulation of secreted proteins involved in migration and invasion. Cell Cycle 14:1961-72 (2015). WB . Read more (PubMed: 26017556) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount 40 µg
Specification Heart, Left Ventricle
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Dawn Bowles

Verified customer

投稿 Dec 28 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"