製品の概要

  • 製品名Anti-Fibronectin antibody [HFN 7.1]
    Fibronectin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HFN 7.1] to Fibronectin
  • 特異性ab80923 is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin. It blocks the fibronectin-medicated cell attachment not by disrupting the collagen-fibronectin interaction, but by interfering with the attachment of fibronectin to its receptor on the cell surface
  • アプリケーション適用あり: Sandwich ELISA, WB, IP, Blockingmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, Human Fibronectin, purified from serum by affinity chromatography on gelatinsepharose.

  • エピトープPeptide core
  • ポジティブ・コントロール
    • SW156 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab80923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibronectin (ab299). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Fibronectin (ab299) as Detection.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 263 kDa.
IP Use at 2 µg/mg of lysate. (Native and denatured - use Protein G)
Blocking Use at an assay dependent dilution.

ターゲット情報

  • 機能Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 組織特異性Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 関連疾患Glomerulopathy with fibronectin deposits 2
  • 配列類似性Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 発生段階Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻訳後修飾Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody [HFN 7.1] 画像

プロトコール

Anti-Fibronectin antibody [HFN 7.1] (ab80923) 使用論文

This product has been referenced in:
  • Ambesi A & McKeown-Longo PJ Conformational remodeling of the fibronectin matrix selectively regulates VEGF signaling. J Cell Sci 127:3805-16 (2014). Read more (PubMed: 24982443) »

See 1 Publication for this product

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Reducing Bovine serum will also put the cells into a lower state of growth with reduced access to growth factors. Growth factor absence will also modulate the activation state of integrin and other cell surf...

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I found a good set of products that gives a strong sandwich ELISA signal.

Capture: ab80923

Detection: ab299

Antigen: ab81743

Click here (or use the following: http://www.abcam.com/index.html?datasheet=80923)....

Read More


I've included a list of capture, detection, protein, and fluorescent-conjugated secondary below against Fibronectin. The capture and detection antibodies have been tested and guaranteed to work in sandwich ELISA to detect human fibronectin:
Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"