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Full length protein, Human Fibronectin, purified from serum by affinity chromatography on gelatinsepharose.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab45688 as a replacement.
Our Abpromise guarantee covers the use of ab80923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibronectin (ab299). For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to Fibronectin (ab299) as Detection.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 263 kDa.|
|IP||Use at 2 µg/mg of lysate. (Native and denatured - use Protein G)|
|Blocking||Use at an assay dependent dilution.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"