製品の概要

  • 製品名Anti-Fibronectin antibody
    Fibronectin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fibronectin
  • 特異性Murine fibronectin 100%, murine collagen type I, III and IV <0.1%, murine laminin <0.1%
  • アプリケーション適用あり: ELISA, RIA, ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Purified fibronectin from murine plasma.

  • ポジティブ・コントロール
    • Mouse skin
  • 特記事項

    This antibody may be used to identify fibronectin during wound healing, during tumour progression, and tumour invasion respectively.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
RIA 1/10000.
ICC/IF 1/40.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 263 kDa).
IHC-P 1/200 - 1/600. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 組織特異性Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 関連疾患Glomerulopathy with fibronectin deposits 2
  • 配列類似性Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 発生段階Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻訳後修飾Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 細胞内局在Secreted, extracellular space, extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CIG antibody
    • Cold insoluble globulin antibody
    • Cold-insoluble globulin antibody
    • DKFZp686F10164 antibody
    • DKFZp686H0342 antibody
    • DKFZp686I1370 antibody
    • DKFZp686O13149 antibody
    • ED B antibody
    • Fibronectin 1 antibody
    • FINC antibody
    • FINC_HUMAN antibody
    • FN antibody
    • FN1 antibody
    • FNZ antibody
    • GFND antibody
    • GFND2 antibody
    • LETS antibody
    • Migration stimulating factor antibody
    • MSF antibody
    • Ugl-Y3 antibody
    see all

Anti-Fibronectin antibody 画像

  • ICC/IF image of ab23750 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23750, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab23750 (2ug/ml) staining Fibronectin in human aorta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining Fibronectin in the cytoplasm and extracellularly in the connective tissue.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab23750 staining fibronectin in mouse liver tissue by immunohistochemistry (frozen sections). Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hours at 4°C. Alexa fluor® 488 goat polyclonal to rabbit Ig, diluted 1/200, was used as the secondary antibody.

    See Abreview

  • ab23750 staining Fibronectin in mouse bone marrow cells by Immunocytochemistry/ Immunofluorescence. Cells were formaldehyde fixed, blocked in 2% BSA for 45 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 9 hour at 4°C. An Alexa Fluor® 488 conjugated goat polyclonal to rabbit IgG, was used undiluted as secondary. The nuclei were stained with DAPI.

  • Immunocytochemical analysis of PFA fixed murine fibroblast tissue sections, labelling Fibronectin with ab23750 at 1/200 incubated for 13 hours at 4°C in TBS with 0.1% BSA. Blocking was with 1% BSA for 1 hour at room temperature. The secondary was a chicken anti-rabbit polyclonal Alexa Fluor® 488 at 1/400.

    See Abreview

  • Anti-Fibronectin antibody (ab23750) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 263 kDa
    Observed band size : 265 kDa (why is the actual band size different from the predicted?)
  • ab23750 staining Fibronectin in Rat skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with Immunoblock® (5 µg/ml) for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in diluent) for 1 hour 30 minutes at 25°C. An undiluted Goat anti-rabbit HRP/DAB polydetector polyclonal was used as the secondary antibody.

    See Abreview

Anti-Fibronectin antibody (ab23750) 使用論文

This product has been referenced in:
  • Kristofik N  et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016). Read more (PubMed: 27471233) »
  • Yang X  et al. VEGF-B promotes cancer metastasis through a VEGF-A-independent mechanism and serves as a marker of poor prognosis for cancer patients. Proc Natl Acad Sci U S A 112:E2900-9 (2015). Read more (PubMed: 25991856) »

See all 27 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Primary Cardiac Fibroblasts)
Permeabilization Yes - Triton x-100, 0.01%
Specification Primary Cardiac Fibroblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Sep 05 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (prostate)
Total protein in input 100 µg
Immuno-precipitation step Other - AminoLink Plus Resin
Specification prostate
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投稿 Nov 05 2015

Application Immunocytochemistry
Sample Mouse Cultured Cells (Fibroblast)
Specification Fibroblast
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Oct 26 2015

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 25 µg
Specification Mouse embryonic fibroblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Sep 30 2015

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Permeabilization Yes - Triton x-100, 0.01%
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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投稿 Jun 17 2015

ELISA

Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (blood)
Specification blood
Type Sandwich (Detection)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Yeri-Alice Rim

Verified customer

投稿 May 23 2013

Thank you for contacting us.

This antibody has been characterised in Western Blot using 5% BSA as a blocking agent (http://www.abcam.com/Fibronectin-antibody-ab23750.html#Fibronectin-Primary-antibodies-ab23750-2.jpg). It has also been reported...

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I can confirm the Safety Data Sheets can be found on the online datasheets. Near the top right hand side of the datasheet, there are two 'buttons', one for Datasheet PDF and the one underneath says SDS. Click on the SDS button to link to the d...

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Thank you for contacting us.

I do understand and appreciate the amount of time you have spent it the laboratory. I am sorry to hear you are disappointed with our service.


If a particular antibody causes problems we do encourag...

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Thank you for taking time to answer my questions.


Having reviewed the additional protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"