Anti-Fibrinopeptide A 抗体 [1F7] (ab14790)

製品の概要

  • 製品名Anti-Fibrinopeptide A antibody [1F7]
    Fibrinopeptide A 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1F7] to Fibrinopeptide A
  • アプリケーション適用あり: ELISA, WB, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Fusion protein:

    ADSGEGDFLAEGGGVR

    , corresponding to N terminal amino acids 1-16 of Human Fibrinopeptide A (alpha chain) and conjugated to a carrier protein.

  • ポジティブ・コントロール
    • IHC-P: FFPE human liver tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab14790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1F3] to Fibrinogen (ab10066).
WB Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Fibrinogen (ab34269). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Fibrinogen (ab34269) as Detection.

ターゲット情報

  • 機能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 組織特異性Plasma.
  • 関連疾患Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
    Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash.
  • 配列類似性Contains 1 fibrinogen C-terminal domain.
  • ドメインA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻訳後修飾The alpha chain is not glycosylated.
    Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
    About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
    Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Phosphorylation sites are present in the extracelllular medium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A alpha polypeptide antibody
    • FGA antibody
    • Fib2 antibody
    • FIBA_HUMAN antibody
    • Fibrinogen alpha chain antibody
    • Fibrinogen alpha/alpha E chain [Precursor] antibody
    see all

Anti-Fibrinopeptide A antibody [1F7] 画像

  • IHC image of Fibrinopeptide A staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14790, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Standard Curve for Fibrinogen (Analyte: Fibrinogen protein (Human) (ab84410)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [1F7] to Fibrinopeptide A (ab14790) at 5µg/ml and Detector Antibody Rabbit polyclonal to Fibrinogen (ab34269) at 0.5µg/ml.

プロトコール

Anti-Fibrinopeptide A antibody [1F7] (ab14790) 使用論文

ab14790 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

Unfortunately we have not determined the epitope for ab14790. We only know that it reacts with fibrinopeptide A region in the alpha-chain of fibrinogen and free fibrinopeptide A.

I am sorry I could not b...

Read More

please find attached to this email the CoC you requested.

Vielen Dank für Ihre Anfrage. In der Tat haben wir einige Antikörper-Paare, die wir für Sandwich-ELISA (sELISA) an humanen Proben garantieren, da die verschiedenen Klone verschiedene Epitope erkennen: Der Klon [1F3] (ab10066) kann mit den Klonen ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein
Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Ewen Smith

Verified customer

投稿 Sep 09 2005

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein
Blocking step Milk as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Ewen Smith

Verified customer

投稿 Sep 06 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"