Anti-Fibrinogen gamma chain 抗体 (ab62527)

製品の概要

  • 製品名Anti-Fibrinogen gamma chain antibody
    Fibrinogen gamma chain 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Fibrinogen gamma chain
  • 特異性ab62527 will bind to the fibrin gamma chain as well as the fibrinogen gamma chain.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 289-338 of Human Fibrinogen gamma chain (AMFKVGPEAD KYRLTYAYFA GGDAGDAFDG FDFGDDPSDK FFTSHNGMQF)

  • ポジティブ・コントロール
    • Fetal liver cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
  • 関連疾患Defects in FGG are a cause of thrombophilia.
    Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen.
  • 配列類似性Contains 1 fibrinogen C-terminal domain.
  • ドメインA long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.
  • 翻訳後修飾Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
    Sulfation of C-terminal tyrosines increases affinity for thrombin.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FGG antibody
    • FIBG_HUMAN antibody
    • Fibrinogen gamma chain antibody
    • Fibrinogen gamma polypeptide antibody
    • fibrinogen gamma-b chain antibody
    see all

Anti-Fibrinogen gamma chain antibody 画像

  • Anti-Fibrinogen gamma chain antibody (ab62527) at 2.5 µg/ml + Fetal liver cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa

Anti-Fibrinogen gamma chain antibody (ab62527) 使用論文

ab62527 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Spleen)
Loading amount 10 µg
Specification Spleen
Treatment FasL for 5 hours
Gel Running Conditions Reduced Non-Denaturing (Native) (4-20%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 May 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"