Anti-Fibrinogen gamma chain 抗体 [5A6] (ab119948)
Key features and details
- Mouse monoclonal [5A6] to Fibrinogen gamma chain
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Fibrinogen gamma chain antibody [5A6]
Fibrinogen gamma chain 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5A6] to Fibrinogen gamma chain -
由来種
Mouse -
アプリケーション
適用あり: WB, ELISA, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to amino acids 210-437 of Human Fibrinogen gamma chain
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ポジティブ・コントロール
- Recombinant Fibrinogen gamma chain protein, NIH3T3 cells
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR240839-12 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
5A6 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119948の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/500 - 1/2000. Predicted molecular weight: 52 kDa.
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ELISA |
1/10000.
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 52 kDa. |
ELISA
1/10000. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
関連疾患
Defects in FGG are a cause of thrombophilia.
Defects in FGG are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. It is a rare autosomal recessive disorder characterized by complete absence of detectable fibrinogen. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
ドメイン
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻訳後修飾
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Sulfation of C-terminal tyrosines increases affinity for thrombin. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2266 Human
- Entrez Gene: 99571 Mouse
- Omim: 134850 Human
- SwissProt: P02679 Human
- SwissProt: Q8VCM7 Mouse
- Unigene: 16422 Mouse
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別名
- FGG antibody
- FIBG_HUMAN antibody
- Fibrinogen gamma chain antibody
see all
画像
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Anti-Fibrinogen gamma chain antibody [5A6] (ab119948) at 1/500 dilution + Recombinant Fibrinogen gamma chain protein
Predicted band size: 52 kDa -
Immunofluorescence analysis of NIH3T3 cells using ab119948 at a dilution of 1/200 (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA, using ab119948 at a dilution of 1/10000.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab119948 は 8 報の論文で使用されています。
- Gustafson KT et al. cyc-DEP: Cyclic immunofluorescence profiling of particles collected using dielectrophoresis. Electrophoresis 43:1784-1798 (2022). PubMed: 35753078
- Hao X et al. The Role of Neutrophil Extracellular Traps in Early Microthrombosis and Brain Injury After Subarachnoid Hemorrhage in Mice. Transl Stroke Res N/A:1-14 (2022). PubMed: 35962915
- Jiang C et al. Fibrinogen promotes gallbladder cancer cell metastasis and extravasation by inducing ICAM1 expression. Med Oncol 40:10 (2022). PubMed: 36352295
- Kuang M et al. FGB and FGG derived from plasma exosomes as potential biomarkers to distinguish benign from malignant pulmonary nodules. Clin Exp Med 19:557-564 (2019). PubMed: 31576477
- Wu L et al. A proteomic analysis identifies candidate early biomarkers to predict ovarian hyperstimulation syndrome in polycystic ovarian syndrome patients. Mol Med Rep 16:272-280 (2017). PubMed: 28534980
- Benito-Jardón M et al. The fibronectin synergy site re-enforces cell adhesion and mediates a crosstalk between integrin classes. Elife 6:N/A (2017). PubMed: 28092265
- Schunke KJ et al. A novel atherothrombotic model of ischemic stroke induced by injection of collagen into the cerebral vasculature. J Neurosci Methods 239:65-74 (2015). PubMed: 25314906
- Liu T et al. Immobilization of heparin/poly-l-lysine nanoparticles on dopamine-coated surface to create a heparin density gradient for selective direction of platelet and vascular cells behavior. Acta Biomater 10:1940-54 (2014). PubMed: 24342042