Biotin Anti-Fibrinogen 抗体 (ab51416)
Key features and details
- Biotin Rabbit polyclonal to Fibrinogen
- Suitable for: ELISA, EIA, IP, RIA, WB
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Fibrinogen antibody
Fibrinogen 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Fibrinogen -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: ELISA, EIA, IP, RIA, WBmore details -
種交差性
交差種: Rat -
免疫原
Full length native protein purified from plasma (Rat)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.50
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51416の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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EIA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 95 kDa.
A band of 340 kDa is seen when run in native gel. |
特記事項 |
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ELISA
Use at an assay dependent concentration. |
EIA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 95 kDa. A band of 340 kDa is seen when run in native gel. |
ターゲット情報
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機能
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
組織特異性
Plasma. -
関連疾患
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
配列類似性
Contains 1 fibrinogen C-terminal domain. -
ドメイン
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
翻訳後修飾
The alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 24366 Rat
- Entrez Gene: 24367 Rat
- Entrez Gene: 361969 Rat
- SwissProt: P02680 Rat
- SwissProt: P06399 Rat
- SwissProt: P14480 Rat
- Unigene: 98846 Rat
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別名
- FGA antibody
- FGB antibody
- FGG antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab51416 は 4 報の論文で使用されています。
- Hu Y et al. The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses. Autophagy 17:1142-1156 (2021). PubMed: 32249716
- Qiao CM et al. Sodium butyrate causes a-synuclein degradation by an Atg5-dependent and PI3K/Akt/mTOR-related autophagy pathway. Exp Cell Res 387:111772 (2020). PubMed: 31836471
- Gan L et al. Reduced Endoplasmic Reticulum Stress-Mediated Autophagy Is Required for Leptin Alleviating Inflammation in Adipose Tissue. Front Immunol 8:1507 (2017). PubMed: 29250056
- Bao H et al. The effects of poloxamer 188 on the autophagy induced by traumatic brain injury. Neurosci Lett 634:7-12 (2016). PubMed: 27693566