Anti-Fibrillarin 抗体 [EPR10822(B)] - Nucleolar Marker (ab154806)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10822(B)] to Fibrillarin - Nucleolar Marker
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker
Fibrillarin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10822(B)] to Fibrillarin - Nucleolar Marker -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human Fibrillarin. The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa, HepG2, 293T and Molt4 cell lysates; Human colonic carcinoma and prostate tissues; HepG2 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10822(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154806の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/250 - 1/500.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/250 - 1/500. |
ターゲット情報
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機能
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). -
配列類似性
Belongs to the methyltransferase superfamily. Fibrillarin family. -
翻訳後修飾
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). -
細胞内局在
Nucleus, nucleolus. Fibrillar region of the nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 2091 Human
- Entrez Gene: 102643269 Mouse
- Entrez Gene: 14113 Mouse
- Entrez Gene: 292747 Rat
- Omim: 134795 Human
- SwissProt: P22087 Human
- SwissProt: P35550 Mouse
- SwissProt: P22509 Rat
see all -
別名
- 34 kD nucleolar scleroderma antigen antibody
- 34 kDa nucleolar scleroderma antigen antibody
- Fbl antibody
see all
画像
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Immunofluorescence analysis of HepG2 cells labeling Fibrillarin with ab154806 at 1/250 dilution.
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All lanes : Anti-Fibrillarin antibody [EPR10822(B)] - Nucleolar Marker (ab154806) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : HepG2 lysate
Lane 3 : 293T lysate
Lane 4 : Molt4 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154806 は 2 報の論文で使用されています。
- Steinbusch MMF et al. Adaptation of the protein translational apparatus during ATDC5 chondrogenic differentiation. Noncoding RNA Res 7:55-65 (2022). PubMed: 35261930
- Luo W et al. Bioinformatic Analysis and In Vitro and In Vivo Experiments Reveal That Fibrillarin Participates in the Promotion of Lung Metastasis in Hepatocellular Carcinoma. Bioengineering (Basel) 9:N/A (2022). PubMed: 36004921