製品の概要

  • 製品名Anti-FHOD3 antibody
  • 製品の詳細
    Rabbit polyclonal to FHOD3
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 390 - 439 (SGKFSGSSPA PPSQPQGLSY AEDAAEHENM KAVLKTSSPS VEDATPALGV) of Human FHOD3 (AAH81563).

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab105651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Actin-organizing protein that may cause stress fiber formation together with cell elongation.
  • 組織特異性Expressed in the heart, kidney and brain.
  • 配列類似性Belongs to the formin homology family.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 細胞内局在Cytoplasm > cytoskeleton. Main part of the protein localizes to actin fibers and the remaining part displays filamentous staining.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ22717 antibody
    • Formactin2 antibody
    • FH1/FH2 domain containing protein 3 antibody
    • FH1/FH2 domain-containing protein 3 antibody
    • Fhod3 antibody
    • FHOD3_HUMAN antibody
    • FHOS2 antibody
    • FLJ22297 antibody
    • Formactin-2 antibody
    • Formin homolog overexpressed in spleen 2 antibody
    • Formin homology 2 domain containing 3 antibody
    • hFHOS2 antibody
    • KIAA1695 antibody
    • mKIAA1695 antibody
    see all

Anti-FHOD3 antibody 画像

  • Anti-FHOD3 antibody (ab105651) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 60 kDa
    The band at 60kD may be either a non-specific protein or a degradation product of the larger ~155-160kD FHOD3 protein. There are no known cleavage products or isoforms known to be around this size. Based on the peptide immunogen used to raise this antibody, this band may be isoform 2 of human CARD14 (434aa; ~82% homology).

    Gel concetration: 12%
  • ab105651, at 8 µg/ml, staining FHOD3 in paraffin embeded Human kidney tissue by Immunohistochemistry. Cells with positive label are the epithelial cells from renal tubules (indicated by arrows). Magnification: 400X.

Anti-FHOD3 antibody (ab105651) 使用論文

This product has been referenced in:
  • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue (Embryonic Heart Cryo-Section)
Permeabilization Yes - Tween-20
Specification Embryonic Heart Cryo-Section
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Verified customer

投稿 Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4%-12%)
Loading amount 10 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Jul 25 2016

Thank you for contacting us.

This band at ˜60kDa has been seen using this product and is on our website (http://www.abcam.com/FHOD3-antibody-ab105651.html#FHOD3-Primary-antibodies-ab105651-1.jpg).The band at 60kD may be either a non-specific...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"