製品の概要

  • 製品名Anti-FHOD3 antibody
  • 製品の詳細
    Rabbit polyclonal to FHOD3
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 390 - 439 (SGKFSGSSPA PPSQPQGLSY AEDAAEHENM KAVLKTSSPS VEDATPALGV) of Human FHOD3 (AAH81563).

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate IHC-P: Human kidney tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab105651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 60 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Actin-organizing protein that may cause stress fiber formation together with cell elongation.
  • 組織特異性Expressed in the heart, kidney and brain.
  • 配列類似性Belongs to the formin homology family.
    Contains 1 DAD (diaphanous autoregulatory) domain.
    Contains 1 FH1 (formin homology 1) domain.
    Contains 1 FH2 (formin homology 2) domain.
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.
  • 細胞内局在Cytoplasm > cytoskeleton. Main part of the protein localizes to actin fibers and the remaining part displays filamentous staining.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ22717 antibody
    • Formactin2 antibody
    • FH1/FH2 domain containing protein 3 antibody
    • FH1/FH2 domain-containing protein 3 antibody
    • Fhod3 antibody
    • FHOD3_HUMAN antibody
    • FHOS2 antibody
    • FLJ22297 antibody
    • Formactin-2 antibody
    • Formin homolog overexpressed in spleen 2 antibody
    • Formin homology 2 domain containing 3 antibody
    • hFHOS2 antibody
    • KIAA1695 antibody
    • mKIAA1695 antibody
    see all

Anti-FHOD3 antibody 画像

  • Anti-FHOD3 antibody (ab105651) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 60 kDa
  • Anti-FHOD3 antibody (ab105651) at 1/1000 dilution + Mouse heart whole tissue lysate at 50 µg

    Secondary
    Goat anti-rabbit IgG-HRP at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 60 kDa
    Observed band size : 160-170 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab105651, at 8 µg/ml, staining FHOD3 in paraffin embeded Human kidney tissue by Immunohistochemistry. Cells with positive label are the epithelial cells from renal tubules (indicated by arrows). Magnification: 400X.

Anti-FHOD3 antibody (ab105651) 使用論文

This product has been referenced in:
  • Lynch J  et al. Metastasis Suppressor microRNA-335 Targets the Formin Family of Actin Nucleators. PLoS One 8:e78428 (2013). WB ; Human . Read more (PubMed: 24223803) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue (Embryonic Heart Cryo-Section)
Permeabilization Yes - Tween-20
Specification Embryonic Heart Cryo-Section
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Jul 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4%-12%)
Loading amount 10 µg
Specification Heart
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 25 2016

Thank you for contacting us.

This band at ˜60kDa has been seen using this product and is on our website (http://www.abcam.com/FHOD3-antibody-ab105651.html#FHOD3-Primary-antibodies-ab105651-1.jpg).The band at 60kD may be either a non-specific...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"