製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FHL2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: YAKKCAGCTN PISGLGGTKY ISFEERQWHN DCFNCKKCSL SLVGRGFLTE, corresponding to a region within C terminal amino acids 217-266 of Human FHL2

  • ポジティブ・コントロール
    • Myocardium

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • 組織特異性
    Expressed in skeletal muscle and heart.
  • 配列類似性
    Contains 4 LIM zinc-binding domains.
  • ドメイン
    The third LIM zinc-binding mediates interaction with E4F1.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

画像

  • Anti-FHL2 antibody (ab66399) at 0.6 µg/ml + Fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • ab66399, at 8µg/ml, staining Human FHL2 in myocardial cells (indicated with arrows) in paraffin-embedded heart tissue, by Immunohistochemistry, 400x magnification.

参考文献

This product has been referenced in:
  • Verset L  et al. Epithelial expression of FHL2 is negatively associated with metastasis-free and overall survival in colorectal cancer. Br J Cancer 109:114-20 (2013). IHC . Read more (PubMed: 23756870) »
  • Matulis CK & Mayo KE The LIM domain protein FHL2 interacts with the NR5A family of nuclear receptors and CREB to activate the inhibin-a subunit gene in ovarian granulosa cells. Mol Endocrinol 26:1278-90 (2012). Human . Read more (PubMed: 22734036) »

See all 3 Publications for this product

レビューと Q&A

Merci de nous avoir contactés.

Le laboratoire qui produit l'anticorps ab66399 m'a confirmé que le démasquage de l'antigène en IHC-P a été effectuéà 98-100°C, en micro-ondes,avec un ta...

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Thank you for contacting us and for your interest in our products. If you want to use the antibody for immunofluorescence on cell culture samples (ICC-IF, immunocytochemistry), I am sorry to confirm that as far as we are aware, ab66399 and ab1...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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