製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FHL2
  • アプリケーション
    適用あり: ICC/IF, IP, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    A synthetic peptide which represented a portion of human FHL2 encoded within exon 8.

  • ポジティブ・コントロール
    • HeLa nuclear extract.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody is affinity purified.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IP Use at 3-10 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 44.6 kDa).

ターゲット情報

  • 機能
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • 組織特異性
    Expressed in skeletal muscle and heart.
  • 配列類似性
    Contains 4 LIM zinc-binding domains.
  • ドメイン
    The third LIM zinc-binding mediates interaction with E4F1.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling FHL2 with ab12328 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 44.6 kDa

    Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12328 used at 0.3 mg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.

    Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12328 used at 0.3 mg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
  • ICC/IF image of ab12328 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12328, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab12328 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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