製品の概要

  • 製品名Anti-FHL2 antibody
    FHL2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FHL2
  • 特異性Reactivity with mouse FHL2 is unlikely owing to divergence of this ortholog from the peptide used for antibody production. The epitope recognized by ab1237 maps to a region between residues 175 and 225 of human Four and a Half LIM domains 2 using the numbering given in entry NP_963849.1 (GeneID 2274).
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A synthetic peptide which represented a portion of human FHL2 encoded within exon 7.

  • ポジティブ・コントロール
    • HeLa nuclear extract.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody is affinity purified.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ICC/IF 1/100.

ターゲット情報

  • 機能May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth.
  • 組織特異性Expressed in skeletal muscle and heart.
  • 配列類似性Contains 4 LIM zinc-binding domains.
  • ドメインThe third LIM zinc-binding mediates interaction with E4F1.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAG 11 antibody
    • AAG11 antibody
    • Aging associated gene 11 antibody
    • Down regulated in rhabdomyosarcoma LIM protein antibody
    • Downregulated in rhabdomyosarcoma LIM protein antibody
    • DRAL antibody
    • FHL 2 antibody
    • FHL-2 antibody
    • Fhl2 antibody
    • FHL2 protein antibody
    • FHL2_HUMAN antibody
    • Four and a half LIM domain protein 2 antibody
    • Four and a half LIM domains 2 antibody
    • Four and a half LIM domains protein 2 antibody
    • KIAA0990 antibody
    • LIM domain protein DRAL antibody
    • Skeletal muscle LIM protein 3 antibody
    • Skeletal muscle LIM-protein 3 antibody
    • SLIM 3 antibody
    • SLIM-3 antibody
    • SLIM3 antibody
    see all

Anti-FHL2 antibody 画像



  • Predicted band size : 32 kDa


    Samples: Nuclear extract (NE) from HeLa cells. Antibody: Affinity purified rabbit anti-FHL2 antibody ab12327 used at 0.3 µg/ml. Detection: Chemiluminescence with an exposure time of 5 minutes.
  • ICC/IF image of ab12327 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12327, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FHL2 antibody (ab12327) 使用論文

This product has been referenced in:
  • Friedrich FW  et al. FHL2 expression and variants in hypertrophic cardiomyopathy. Basic Res Cardiol 109:451 (2014). Read more (PubMed: 25358972) »
  • Gökmen-Polar Y  et al. Dual targeting of EphA2 and ER restores tamoxifen sensitivity in ER/EphA2-positive breast cancer. Breast Cancer Res Treat 127:375-84 (2011). WB ; Human . Read more (PubMed: 20602165) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Permeabilization Yes - saponin
Specification Human pluripotent stem cell derived cardiomyocyte
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 21 2015

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Mouse Cell lysate - whole cell (bone marrow cells)
Specification bone marrow cells
Treatment 30 ng/ml RANKL and 30 ng/ml MCSF for 5 days
Total protein in input 2000 µg
Username

Do Yu Soung

Verified customer

投稿 Jul 26 2013

Le peptide bloquant de l'anti-FHL2 ab12327 est maintenant disponible à la vente sous la référence ab119360. La fiche technique est disponible via le lien www.abcam.com/ab119360 . J'espère que ces informations vous seront utiles. N'hésitez pas à nous...

Read More

Le laboratoire m'a informé que le peptide bloquant est disponible à la vente, il sera ajouté au catalogue dans la journée d'aujourd'hui ou au plus tard demain. Je vous enverrai la référence de ce produit quand la fiche technique aura été créée. La s...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (osteoblast)
Specification osteoblast
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 29 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"