製品の概要

  • 製品名Anti-FHIT antibody
    FHIT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FHIT
  • 特異性FHIT antibody detects endogenous levels of total FHIT protein.
  • アプリケーション適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide derived from human FHIT

  • ポジティブ・コントロール
    • Human breast carcinoma; A549 cell extract.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 関連性FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • AP3A hydrolase antibody
    • AP3A hydrolase fragile site 3p14.2 antibody
    • AP3Aase antibody
    • Bis 5' adenosyl triphosphatase antibody
    • Bis(5' adenosyl) triphosphatase antibody
    • Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody
    • Dinucleosidetriphosphatase antibody
    • FRA 3B antibody
    • FRA3B antibody
    • Fragile histidine triad antibody
    • Fragile histidine triad gene antibody
    • Fragile histidine triad protein antibody
    • Tumor suppressor protein antibody
    see all

Anti-FHIT antibody 画像

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue
    using ab53074 at 1/50 dilution, with and without peptide immunogen.
  • All lanes : Anti-FHIT antibody (ab53074) at 1/500 dilution

    Lane 1 : A549 cell extract with no immunizing peptide
    Lane 2 : A549 cell extract with immunizing peptide


    Predicted band size : 17 kDa
    Observed band size : 17 kDa
  • ICC/IF image of ab53074 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53074, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-FHIT antibody (ab53074) 使用論文

ab53074 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am pleased to provide the testing protocol below, including the antigen retreival. I hope this will be helpful. Please note this is a guideline only, and may require some further individual optimization by the end...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (lymphocyte)
Specification lymphocyte
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Julian Laubental

Verified customer

投稿 Apr 20 2012

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this...

Read More

Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from this antibody.

The details your customer has kindly provided...

Read More

Thank you for your patient. The immunogen peptide sequence range is amino acids 110-130. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"