製品の概要

  • 製品名Anti-FHIT antibody
    FHIT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to FHIT
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human).

  • ポジティブ・コントロール
    • Normal Breast IF/ICC: HCT116 cell line.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
IHC-P
  • 追加情報IHC-P: 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性FHIT (fragile histidine triad) cleaves adenosine 5' PPP 5' A to yield AMP and ADP. Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor. FHIT physically associates with ubiquitin conjugating enzyme 9.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • AP3A hydrolase antibody
      • AP3A hydrolase fragile site 3p14.2 antibody
      • AP3Aase antibody
      • Bis 5' adenosyl triphosphatase antibody
      • Bis(5' adenosyl) triphosphatase antibody
      • Diadenosine 5' 5''' P1 P3 triphosphate hydrolase antibody
      • Dinucleosidetriphosphatase antibody
      • FRA 3B antibody
      • FRA3B antibody
      • Fragile histidine triad antibody
      • Fragile histidine triad gene antibody
      • Fragile histidine triad protein antibody
      • Tumor suppressor protein antibody
      see all

    Anti-FHIT antibody 画像

    • ICC/IF image of ab15287 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15287, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-FHIT antibody (ab15287) 使用論文

    This product has been referenced in:
    • Chava S  et al. Immunohistochemical evaluation of p53, FHIT, and IGF2 gene expression in esophageal cancer. Dis Esophagus 25:81-7 (2012). IHC-P ; Human . Read more (PubMed: 21668571) »
    • Czyzewska J  et al. Immunohistochemical assessment of Fhit protein expression in advanced gastric carcinomas in correlation with Helicobacter pylori infection and survival time. Folia Histochem Cytobiol 47:47-53 (2009). Read more (PubMed: 19419937) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab15287.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"