Anti-FH 抗体 [8F12BB5] (ab110286)

製品の概要

  • 製品名Anti-FH antibody [8F12BB5]
    FH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8F12BB5] to FH
  • アプリケーション適用あり: In-Cell ELISA, IP, IHC-P, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Rat, Cow, Human
  • 免疫原

    Purified FH proteins from bovine liver, bovine heart, porcine heart

  • ポジティブ・コントロール
    • Human HDFn cells; Human cerebellum tissue; HepG2 cells, human liver mitochondria, bovine liver mitochondria, and rat liver mitochondria; HeLa cells
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
In-Cell ELISA Use a concentration of 4 µg/ml. 0.4 µg/well
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. Recommend 0.1% Triton X-100 permeabilization.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Also acts as a tumor suppressor.
  • パスウェイCarbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.
  • 関連疾患Defects in FH are the cause of fumarase deficiency (FHD) [MIM:606812]; also known as fumaricaciduria. FHD is characterized by progressive encephalopathy, developmental delay, hypotonia, cerebral atrophy and lactic and pyruvic acidemia.
    Defects in FH are the cause of multiple cutaneous and uterine leiomyomata (MCUL1) [MIM:150800]. MCUL1 is an autosomal dominant condition in which affected individuals develop benign smooth muscle tumors (leiomyomata) of the skin. Affected females also usually develop leiomyomata of the uterus (fibroids).
    Defects in FH are the cause of hereditary leiomyomatosis and renal cell cancer (HLRCC) [MIM:605839].
  • 配列類似性Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.
  • 細胞内局在Cytoplasm and Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FH antibody
    • Fumarase antibody
    • Fumarate hydratase antibody
    • Fumarate hydratase mitochondrial antibody
    • Fumarate hydratase, mitochondrial antibody
    • FUMH_HUMAN antibody
    • HLRCC antibody
    • LRCC antibody
    • MCL antibody
    • MCUL 1 antibody
    • MCUL1 antibody
    • MS709 antibody
    • Multiple hereditary cutaneous leiomyomata antibody
    see all

Anti-FH antibody [8F12BB5] 画像

  • HeLa cells were stained with 1 µg/mL ab110286 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • Immunocytochemistry image of FH (ab110286) stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110286 at 5µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
  • FH immunocaptured from HepG2 cells (Lane 1), human liver mitochondria (Lane 2), bovine liver mitochondria (Lane 3), and rat liver mitochondria (Lane 4) using ab110286.
  • ab110286 staining FH in formalin-fixed, paraffin-embedded human cerebellum tissue by Immunohistochemistry at 5ug/ml. FH immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

Anti-FH antibody [8F12BB5] (ab110286) 使用論文

ab110286 has not yet been referenced specifically in any publications.

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