製品の概要

  • 製品名Anti-FGGY antibody
    FGGY 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to FGGY
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Recombinant fragment: MWLDHRAVSQ VNRINETKHS VLQYVGGVMS VEMQAPKLLW LKENLREICW DKAGHFFDLP DFLSWKATGV TARSLCSLVC KWTYSAEKGW DDSFWKMIG, corresponding to amino acids 113-211 of Human FGGY carbohydrate kinase domain containing (NP_060761) with proprietary tag.

  • ポジティブ・コントロール
    • HeLa cell lysate; Recombinant tagged human FGGY carbohydrate kinase domain containing fragment.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 組織特異性Expressed in kidney, lung and small intestine and to a lower extent in liver and detected in cerebrospinal fluid (at protein level).
  • 関連疾患Defects in FGGY are associated with sporadic amyotrophic lateral sclerosis (ALS) [MIM:105400]. Amyotrophic lateral sclerosis is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors.
  • 配列類似性Belongs to the FGGY kinase family.
  • 発生段階Expressed in fetal brain (at protein level).
  • Information by UniProt
  • 参照データベース
  • 別名
    • fggy antibody
    • FGGY carbohydrate kinase domain containing antibody
    • FGGY carbohydrate kinase domain-containing protein antibody
    • FGGY_HUMAN antibody
    • FLJ10986 antibody
    • MGC94804 antibody
    • OTTHUMP00000010078 antibody
    • OTTHUMP00000010081 antibody
    • OTTHUMP00000010082 antibody
    • OTTHUMP00000202071 antibody
    • RP11-242B9.1 antibody
    see all

Anti-FGGY antibody 画像

  • Anti-FGGY antibody (ab77723) at 1 µg/ml + Recombinant tagged human FGGY carbohydrate kinase domain containing fragment at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 60 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-FGGY antibody (ab77723) at 1 µg/ml + HeLa cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

Anti-FGGY antibody (ab77723) 使用論文

ab77723 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T)
Loading amount 25 µg
Specification HepG2, 293T
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Aug 27 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (MEF, C2C12)
Loading amount 25 µg
Specification MEF, C2C12
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"