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Synthetic peptide corresponding to Human FGFR4 aa 24-55 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab5481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 87 kDa).|
|IHC-P||1/50 - 1/100.|
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FGFR4 with ab5481 at 1/200. Cells were fixed with 4% paraformaldehyde for 20 minutes and permeabilized with 0.2% Triton X-100 for 30 minutes. Cells were incubated with the primary antibody for 2 hours at room temperature. An Alexa Fluor® 488 conjugated donkey anti-rabbit IgG (green) was used as the secondary antibody (1/1000, 1 hour). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 minutes).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling FGFR4 with ab5481. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.
Flow cytometry analysis of WiDr cells labelling FGFR4 (green) with ab5481 compared to a negative control. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody.