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Our Abpromise guarantee covers the use of ab44971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/50. See Abreview.
ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC||Use at an assay dependent concentration.|
ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.