製品の概要
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製品名Anti-FGFR4 antibody [5B5]
FGFR4 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [5B5] to FGFR4
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由来種Mouse
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特異性ab44971 detects fibroblasts.
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アプリケーション適用あり: IHC-Fr, Flow Cyt, ICC, ICC/IF, IHC-Pmore details
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種交差性交差種: Mouse, Human
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免疫原
Fusion protein corresponding to Human FGFR4.
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ポジティブ･コントロール
- ICC/IF: Human BJ fibroblasts; mouse primary airway cells. IHC-Fr: Human skin fibroblasts. Flow: Human primary adult fibroblasts. IHC-P: Murine abdominal fibroblasts.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading... -
精製度Protein G purified
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ポリ/モノモノクローナル
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クローン名5B5
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
アプリケーション
Our Abpromise guarantee covers the use of ab44971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | 1/50. | |
| Flow Cyt | 1/50. See Abreview.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| ICC | Use at an assay dependent concentration. | |
| ICC/IF | 1/50. | |
| IHC-P | 1/30. |
ターゲット情報
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機能Receptor for acidic fibroblast growth factor. Does not bind to basic fibroblast growth factor. Binds FGF19.
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組織特異性Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
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配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾Glycosylated.
Phosphorylated on tyrosine residue (By similarity). Phosphorylation requires the presence of a functional (phosphorylated) FGFR1 and not necessarily by means of FGFR heterodimerization. -
細胞内局在Membrane. Isoform 2 may be secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 2264 Human
- Entrez Gene: 14186 Mouse
- Omim: 134935 Human
- SwissProt: P22455 Human
- SwissProt: Q03142 Mouse
- Unigene: 165950 Human
- Unigene: 276715 Mouse
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別名
- CD 334 antibody
- CD334 antibody
- CD334 antigen antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-FGFR4 antibody [5B5] (ab44971)](http://a.abcam.com/ps/products/44/ab44971/Images/ab44971-rev9391-orig.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGFR4 antibody [5B5] (ab44971)This image is courtesy of an anonymous Abreviewab44971 (1/50) detecting fibroblast cells (red). Human BJ fibroblast cells were fixed in formaldehyde, permeabilized in 0.1% Triton and blocked in 8% BSA. -
![Immunohistochemistry (Frozen sections) - Anti-FGFR4 antibody [5B5] (ab44971)](http://a.abcam.com/ps/products/44/ab44971/Images/Fibroblast-Primary-antibodies-ab44971-2.jpg)
Immunohistochemistry (Frozen sections) - Anti-FGFR4 antibody [5B5] (ab44971)ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in normal human skin tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 10% BSA for 30 minutes at 21°C. The primary antibody was diluted 1/50 in PBT (PBS + 0.5% Tween 20 + 0.5% BSA) and incubated with sample for 30 minutes at 21°C. A HRP conjugated goat polyclonal to mouse IgG was used undiluted as secondary.
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![Flow Cytometry - Anti-FGFR4 antibody [5B5] (ab44971)](http://a.abcam.com/ps/products/44/ab44971/Images/Fibroblast-Primary-antibodies-ab44971-6.jpg)
Flow Cytometry - Anti-FGFR4 antibody [5B5] (ab44971)This image is courtesy of an anonymous Abreviewab44971 staining Human primary adult fibroblasts by Flow Cytometry. Cells were harvested with 1 min 0.05% trypsin treatment at 37°C in 5% FBS. Cells were fixed with paraformaldehyde and permeabilized with Triton X-100. The sample was incubated with the primary antibody, (1/50 in 5% FBS), for 20 minutes at 21°C. An Alexa Fluor®647-conjugated Goat polyclonal to mouse IgG (1/400) was used as secondary antibody. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR4 antibody [5B5] (ab44971)](http://a.abcam.com/ps/products/44/ab44971/Images/Fibroblast-Primary-antibodies-ab44971-9.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGFR4 antibody [5B5] (ab44971)Image from Liao IC et al, Biomaterials. 2011 Feb;32(6):1669-77. Epub 2010 Nov 16, Fig 3. DOI 10.1016/j.biomaterials.2010.10.049ab44971 Mouse monoclonal [5B5] to FGFR4 staining fibroblasts in murine abdominal tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). -
![Immunocytochemistry/ Immunofluorescence - Anti-FGFR4 antibody [5B5] (ab44971)](http://a.abcam.com/ps/products/44/ab44971/Images/ab44971-308179-9711.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGFR4 antibody [5B5] (ab44971)Formaldehyde-fixed, 5% Triton 100X permeabilized mouse primary airway cells labeling FGFR4 with ab44971 at 1/300 dilution in ICC/IF. 4% M.O.M Ig Blocking was used as a blocking agent for 1 hour at 20°C. Primary antibody was incubated at 4°C for 16 hours. Secondary antibody used was a biotin-conjugated rabbit polyclonal anti-mouse Ig at 1/100 dilution.
プロトコール
参考文献
This product has been referenced in:
- Chen G et al. Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice. Brain 137:2193-209 (2014). ICC/IF ; Mouse . Read more (PubMed: 24919967) »
- Carmona R et al. Cells derived from the coelomic epithelium contribute to multiple gastrointestinal tissues in mouse embryos. PLoS One 8:e55890 (2013). ICC/IF ; Mouse . Read more (PubMed: 23418471) »
レビューと Q&A
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