製品の概要

  • 製品名Anti-FGFR1 antibody [EPR806Y]
    FGFR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR806Y] to FGFR1
  • アプリケーション適用あり: ICC/IF, WBmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human FGFR1 aa 800 to the C-terminus (C terminal).
    (Peptide available as ab177436)

  • ポジティブ・コントロール
    • WB: HAP1, FGFR1 knockout HAP1, MCF-7 and SH-SY5Y cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/500. Predicted molecular weight: 92 kDa.Can be blocked with FGFR1 peptide (ab177436).
  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Receptor for basic fibroblast growth factor. Receptor for FGF23 in the presence of KL (By similarity). A shorter form of the receptor could be a receptor for FGF1 (aFGF).
    • 組織特異性Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells.
    • 関連疾患Defects in FGFR1 are a cause of Pfeiffer syndrome (PS) [MIM:101600]; also known as acrocephalosyndactyly type V (ACS5). PS is characterized by craniosynostosis (premature fusion of the skull sutures) with deviation and enlargement of the thumbs and great toes, brachymesophalangy, with phalangeal ankylosis and a varying degree of soft tissue syndactyly.
      Defects in FGFR1 are a cause of idiopathic hypogonadotropic hypogonadism (IHH) [MIM:146110]. IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function.
      Defects in FGFR1 are the cause of Kallmann syndrome type 2 (KAL2) [MIM:147950]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. In some cases, midline cranial anomalies (cleft lip/palate and imperfect fusion) are present and anosmia may be absent or inconspicuous.
      Defects in FGFR1 are the cause of osteoglophonic dysplasia (OGD) [MIM:166250]; also known as osteoglophonic dwarfism. OGD is characterized by craniosynostosis, prominent supraorbital ridge, and depressed nasal bridge, as well as by rhizomelic dwarfism and nonossifying bone lesions. Inheritance is autosomal dominant.
      Defects in FGFR1 are the cause of trigonocephaly non-syndromic (TRICEPH) [MIM:190440]; also known as metopic craniosynostosis. The term trigonocephaly describes the typical keel-shaped deformation of the forehead resulting from premature fusion of the frontal suture. Trigonocephaly may occur also as a part of a syndrome.
      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell leukemia lymphoma syndrome (SCLL). Translocation t(8;13)(p11;q12) with ZMYM2. SCLL usually presents as lymphoblastic lymphoma in association with a myeloproliferative disorder, often accompanied by pronounced peripheral eosinophilia and/or prominent eosinophilic infiltrates in the affected bone marrow.
      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(6;8)(q27;p11) with FGFR1OP. Insertion ins(12;8)(p11;p11p22) with FGFR1OP2. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion proteins FGFR1OP2-FGFR1, FGFR1OP-FGFR1 or FGFR1-FGFR1OP may exhibit constitutive kinase activity and be responsible for the transforming activity.
      Note=A chromosomal aberration involving FGFR1 may be a cause of stem cell myeloproliferative disorder (MPD). Translocation t(8;9)(p12;q33) with CEP110. MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma. In general it progresses to acute myeloid leukemia. The fusion protein CEP110-FGFR1 is found in the cytoplasm, exhibits constitutive kinase activity and may be responsible for the transforming activity.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Fibroblast growth factor receptor subfamily.
      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Binding of FGF1 and heparin promotes autophosphorylation on tyrosine residues and activation of the receptor.
    • 細胞内局在Membrane. Nucleus. Cytoplasm. Cytoplasmic vesicle
    • Information by UniProt
    • 参照データベース
    • 別名
      • Basic fibroblast growth factor receptor 1 antibody
      • bFGF-R-1 antibody
      • BFGFR antibody
      • CD331 antibody
      • CEK antibody
      • FGFBR antibody
      • FGFR 1 antibody
      • FGFR-1 antibody
      • FGFR1 antibody
      • FGFR1/PLAG1 fusion antibody
      • FGFR1_HUMAN antibody
      • fibroblast growth factor receptor 1 antibody
      • FLG antibody
      • FLT-2 antibody
      • FLT2 antibody
      • Fms-like gene antibody
      • Fms-like tyrosine kinase 2 antibody
      • fms-related tyrosine kinase 2 antibody
      • HBGFR antibody
      • heparin-binding growth factor receptor antibody
      • HH2 antibody
      • HRTFDS antibody
      • hydroxyaryl-protein kinase antibody
      • KAL2 antibody
      • N-SAM antibody
      • OGD antibody
      • Proto-oncogene c-Fgr antibody
      see all

    Anti-FGFR1 antibody [EPR806Y] 画像



    • Predicted band size : 92 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: FGFR1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: MCF7 whole cell lysate (20 µg)
      Lane 4: SH-SY5Y whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab76464 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab76464 was shown to specifically react with FGFR1 when FGFR1 knockout samples were used. Wild-type and FGFR1 knockout samples were subjected to SDS-PAGE.  ab76464 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/mL and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

       

    • Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution (purified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 92 kDa
      Observed band size : 145 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-FGFR1 antibody [EPR806Y] (ab76464) at 1/500 dilution (unpurified) + SH-SY5Y cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 92 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    Anti-FGFR1 antibody [EPR806Y] (ab76464) 使用論文

    This product has been referenced in:
    • Zhang J  et al. Whole-genome sequencing identifies genetic alterations in pediatric low-grade gliomas. Nat Genet 45:602-12 (2013). WB . Read more (PubMed: 23583981) »
    • Zhang J  et al. Translating the therapeutic potential of AZD4547 in FGFR1-amplified non-small cell lung cancer through the use of patient-derived tumor xenograft models. Clin Cancer Res 18:6658-67 (2012). Read more (PubMed: 23082000) »

    See all 3 Publications for this product

    Product Wall

    Thank you for contacting us.

    Please search in literature for evidence about the negativity of cell lines. NCI-H1703 does express FGFR3.

    http://rcgdb.bioinf.uni-sb.de/MutomeWeb/SimpleSampleQuery?query=NCI-H1703

    http://www.ebi....

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"